Amphiphilic Porphyrins for Second Harmonic Generation Imaging
Amphiphilic Porphyrins for Second Harmonic Generation Imaging
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DOI：
10.1021/ja8061369
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发表时间：
2009-03-04
影响因子：
15
通讯作者：
Anderson, Harry L.
中科院分区：
文献类型：
--
作者：
Reeve, James E.;Collins, Hazel A.;Anderson, Harry L.
两亲性的供体-受体meso-乙炔基卟啉与极性吡啶鎓电子受体头基和疏水性的二烷基-苯胺电子供体具有高分子超极化率（如通过超瑞利散射测量）和生物膜的高亲和力。当与十二烷中的水滴或活细胞的质膜结合时，它们可用于二次谐波发生（SHG）显微镜;波长为840 nm的入射光在420 nm处产生强烈的倍频信号。铜（II）和镍（II）卟啉配合物给出类似的SHG信号的游离碱卟啉，而表现出没有可检测的双光子激发荧光。
Amphiphilic donor-acceptor meso-ethynyl porphyrins with polar pyridinium electron-acceptor head groups and hydrophobic dialkyl-aniline electron donors have high molecular hyperpolarizabilities (as measured by hyper-Rayleigh scattering) and high affinities for biological membranes. When bound to water droplets in dodecane, or to the plasma membranes of living cells, they can be used for second harmonic generation (SHG) microscopy; an incident light of wavelength 840 nm generates a strong frequency-doubled signal at 420 nm. Copper(II) and nickel(II) porphyrin complexes give similar SHG signals to those of the free-base porphyrins, while exhibiting no detectable two-photon excited fluorescence.