Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion.
Three-Dimensional (3D) Printed Microneedles for Microencapsulated Cell Extrusion.
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DOI：
10.3390/bioengineering5030059
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发表时间：
2018-07-31
期刊：
影响因子：
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通讯作者：
Mobed-Miremadi M
中科院分区：
文献类型：
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作者：
Farias C;Lyman R;Hemingway C;Chau H;Mahacek A;Bouzos E;Mobed-Miremadi M
基于细胞-水凝胶的疗法为伤口愈合提供了巨大的希望。本研究的具体目的是评估人肝癌（HepG2）细胞在雾化海藻酸盐胶囊（3.5% （w/v）海藻酸盐，d = 225µm±24.5µm）中，通过立体光刻制作的三维（3D）打印甲基丙烯酸酯为基础的定制中空微针组件（13个锥形锥的圆形阵列）挤压后的活力。挤出喷嘴尖端（d = 325 μm）的根均方粗糙度为158 nm的溶剂灭菌装置以12 mL/min的流速运行，喷射可靠性为80%。挤压时间为2 h （p = 0.14, α = 0.05）和24 h （p = 0.5, α = 0.05）时，剪切后样品的活力与对照无显著差异。考虑到水凝胶生物侵蚀导致的挤压率从21.2%增加到56.4%（可通过弹性损失从5470 （J/m3）减少到3250 （J/m3）来量化），与雾化后2小时（9.9±2.8%）相比，挤压发生24小时（12.2±4.9%）时的相对有效载荷百分比（p = 0.2628, α = 0.05）没有显著差异。本文的结果强调了胶囊细胞挤压的可行性，特别是保护免受剪切，通过一个空心微针组件首次报道在文献中。
Cell-hydrogel based therapies offer great promise for wound healing. The specific aim of this study was to assess the viability of human hepatocellular carcinoma (HepG2) cells immobilized in atomized alginate capsules (3.5% (w/v) alginate, d = 225 µm ± 24.5 µm) post-extrusion through a three-dimensional (3D) printed methacrylate-based custom hollow microneedle assembly (circular array of 13 conical frusta) fabricated using stereolithography. With a jetting reliability of 80%, the solvent-sterilized device with a root mean square roughness of 158 nm at the extrusion nozzle tip (d = 325 μm) was operated at a flowrate of 12 mL/min. There was no significant difference between the viability of the sheared and control samples for extrusion times of 2 h (p = 0.14, α = 0.05) and 24 h (p = 0.5, α = 0.05) post-atomization. Factoring the increase in extrusion yield from 21.2% to 56.4% attributed to hydrogel bioerosion quantifiable by a loss in resilience from 5470 (J/m3) to 3250 (J/m3), there was no significant difference in percentage relative payload (p = 0.2628, α = 0.05) when extrusion occurred 24 h (12.2 ± 4.9%) when compared to 2 h (9.9 ± 2.8%) post-atomization. Results from this paper highlight the feasibility of encapsulated cell extrusion, specifically protection from shear, through a hollow microneedle assembly reported for the first time in literature.