Forced treadmill exercise prevents oxidative stress and memory deficits following chronic cerebral hypoperfusion in the rat
Forced treadmill exercise prevents oxidative stress and memory deficits following chronic cerebral hypoperfusion in the rat
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DOI：
10.1016/j.nlm.2011.09.008
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发表时间：
2012-01-01
影响因子：
2.7
通讯作者：
Netto, Carlos Alexandre
中科院分区：
文献类型：
--
作者：
Cechetti, Fernanda;Worm, Paulo Valdeci;Netto, Carlos Alexandre
体力活动影响人类中风后的功能恢复，但其对慢性脑灌注不足的实验动物的影响尚未研究。本研究的目的是通过测量大分子损伤、TBARS水平和总细胞硫醇以及海马、纹状体和大脑皮层中的抗氧化酶，通过Morris水迷宫中的认知活动和脑氧化状态评估运动的治疗潜力。成年雄性Wistar大鼠接受改良的永久性双侧颈总动脉闭塞（2 VO）方法，首先闭塞右侧颈总动脉，并在缺血事件后3个月进行测试。三种不同的运动方案的影响进行了检查：缺血前，缺血后和缺血前+缺血后。体育锻炼包括20分钟，每周3次，持续12周（中等强度）。在参考和工作空间记忆中对大鼠进行认知评估，并在最后一次测试后处死大鼠以测定氧化应激参数。低灌注引起了显着的认知缺陷，在两个空间水迷宫任务，这种影响被逆转后，接受运动方案后和前+ -后缺血事件的大鼠。此外，强迫定期跑步机运动调节海马体中的氧化损伤和抗氧化酶活性。这些结果表明，体育锻炼可以防止慢性脑灌注不足引起的认知和生化障碍。(C)2011 Elsevier Inc. All rights reserved.
Physical activity impacts functional recovery following stroke in humans, however its effects in experimental animals submitted to chronic cerebral hypoperfusion have not been investigated. The aim of this study was to evaluate the therapeutic potential of exercise, as assessed by cognitive activity in the Morris water maze and the brain oxidative status, through measurement of macromolecules damage, TBARS levels and total cellular thiols, as well as antioxidant enzymes in hippocampus, striatum and cerebral cortex. Adult male Wistar rats were submitted to the modified permanent bilateral occlusion of the common carotid arteries (2VO) method, with right common carotid artery being first occluded, and tested 3 months after the ischemic event. The effects of three different exercise protocols were examined: pre-ischemia, post-ischemia and pre + post-ischemia. Physical exercise consisted of sessions of 20-min, 3 times per week during 12 weeks (moderate intensity). Rats were submitted to cognitive assessment, in both reference and working spatial memory and after the last testing session were sacrificed to have oxidative stress parameters determined. Hypoperfusion caused a significant cognitive deficit in both spatial water maze tasks and this effect was reversed in rats receiving exercise protocol post and pre + -post the ischemic event. Moreover, forced regular treadmill exercise regulated oxidative damage and antioxidant enzyme activity in the hippocampus. These results suggest that physical exercise protects against cognitive and biochemical impairments caused by chronic cerebral hypoperfusion. (C) 2011 Elsevier Inc. All rights reserved.