Reduced expression of the complement receptor type 2 (CR2, CD21) by synovial fluid B and T lymphocytes
Reduced expression of the complement receptor type 2 (CR2, CD21) by synovial fluid B and T lymphocytes
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DOI：
10.1046/j.1365-2249.2000.01379.x
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发表时间：
2000-11-01
影响因子：
4.6
通讯作者：
Melchers, I
中科院分区：
文献类型：
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作者：
Illges, H;Braun, M;Melchers, I
用细胞荧光法和逆转录聚合酶链反应分析了不同类型关节炎患者滑膜B淋巴细胞和T淋巴细胞中CR2 （CD21）的表达。CR2 （CD21）细胞表面蛋白在外周血B细胞中检测到正常数量，但在同一患者的滑膜B淋巴细胞中几乎没有检测到。这种减少在类风湿性关节炎患者中最为严重，但在所有其他病例中也观察到。体外培养后未出现CR2 （CD21）。CR2 (CD21) mRNA在滑膜B淋巴细胞和T淋巴细胞中也明显降低。滑液B淋巴细胞大于外周血B淋巴细胞，而同一患者的T细胞大小无差异。我们得出结论，滑膜液B淋巴细胞经历了一个不可逆转的终末分化的步骤。外周T细胞和滑膜T细胞中CR2 (CD21) mRNA的存在或缺失表明，CR2 （CD21）在T淋巴细胞中的表达也存在差异。
The expression of CR2 (CD21) by synovial B and T lymphocytes of patients suffering from various forms of arthritis was analysed with cytofluorometry and with reverse transcriptase-polymerase chain reaction. CR2 (CD21) cell surface protein was detected in normal quantities on peripheral B cells, but was almost absent on synovial B lymphocytes of the same patients. This reduction was most severe in patients with rheumatoid arthritis, but also observed in all other cases. CR2 (CD21) did not reappear after in vitro culture. CR2 (CD21) mRNA was also strongly reduced in synovial B and T lymphocytes. Synovial fluid B lymphocytes were larger than peripheral blood B lymphocytes, while T cells from the same patients showed no size differences. We conclude that synovial fluid B lymphocytes have undergone an irreversible step towards terminal differentiation. The presence or absence of CR2 (CD21) mRNA in peripheral versus synovial T cells indicates that CR2 (CD21) is also differentially expressed by T lymphocytes.