Antisense DNA downregulates protein kinase C isozymes (beta and alpha) and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes.
Antisense DNA downregulates protein kinase C isozymes (beta and alpha) and insulin-stimulated 2-deoxyglucose uptake in rat adipocytes.
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反义 DNA 下调大鼠脂肪细胞中蛋白激酶 C 同工酶（β 和 α）和胰岛素刺激的 2-脱氧葡萄糖摄取。
DOI：
10.1089/ard.1991.1.35
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Wickstrom,E
中科院分区：
文献类型：
--
作者：
Farese,RV;Standaert,ML;Ishizuka,T;Yu,B;Hernandez,H;Waldron,C;Watson,J;Farese,JP;Cooper,DR;Wickstrom,E
用与蛋白激酶C (PKC) α和β同工酶mRNA起始密码子区互补的反义二甲氧基三烷基五十氧核苷酸处理大鼠脂肪细胞。这种反义处理引起PKC和胰岛素刺激的2-脱氧葡萄糖摄取减少50-70%，但不抑制胰岛素刺激的二酰基甘油合成。有意义或无意义的寡脱氧核苷酸对PKC和2-脱氧葡萄糖摄取没有影响。这些结果表明：(1)PKC-α和PKC-β同工酶在大鼠脂肪细胞中可通过反义寡脱氧核苷酸特异性下调；(2)胰岛素刺激的葡萄糖转运需要PKC。
Rat adipocytes were treated with antisense dimethoxytrityl pentadecadeoxynucleotides, complementary to mRNA initiation codon regions for α and β isozymes of protein kinase C (PKC). This antisense treatment provoked 50–70% decreases in PKC and insulin-stimulated 2-deoxyglucose uptake, but did not inhibit insulin-stimulated diacylglycerol synthesis. Sense or nonsense oligodeoxynucleotides were without effect on PKC and 2-deoxyglucose uptake. These results suggest that: (i) PKC-α and PKC-β isozymes can be specifically downregulated in rat adipocytes by antisense oligodeoxynucleotides, and (ii) insulin-stimulated glucose transport requires PKC.