Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures.
Cytotoxicity of 35 dental resin composite monomers/additives in permanent 3T3 and three human primary fibroblast cultures.
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DOI：
10.1002/(sici)1097-4636(19980905)41:3
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发表时间：
1998-09
期刊：
影响因子：
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通讯作者：
W. Geurtsen;F. Lehmann;W. Spahl;G. Leyhausen
中科院分区：
文献类型：
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作者：
W. Geurtsen;F. Lehmann;W. Spahl;G. Leyhausen
本研究的目的是确定35种单体或添加剂在商用牙科树脂复合材料中的细胞毒性作用（ED50浓度）。永久性3T3细胞单层和来源于口腔组织（牙龈、牙髓和牙周韧带）的三种主要人类成纤维细胞类型被用作测试系统。所有物质的测试浓度范围为0.01至5.0 mM。一般来说，ED50值在0.06至0.5 mM之间变化。在co（单体）、引发剂和促生剂组中，可以评估严重（如bi - gma、UDMA、DMBZ和DMDTA）或中度（HEMA、BEMA、CQ、DMPT和DMAPE）的细胞毒性作用。在反应/分解产物组中，仅发现中度或轻微的影响（ED50: 0.7至bbb50 mM）。然而，抑制剂BHT、污染物TPSb和光稳定剂HMBP在所有细胞培养中都具有高度的细胞毒性。此外，DBPO和HMBP的ED50值随细胞培养量的增加而显著变化（分别为0.43 ~ 3.8 mM和0.44 ~ 3.07 mM）。我们的综合筛选表明，对于一些高细胞毒性的复合成分，细胞毒性较小的替代品是可用的。此外，没有发现哪种类型的细胞对测试化合物的毒性作用比其他类型的细胞更低或更敏感。然而，与3T3和牙龈成纤维细胞相比，人类原代牙周韧带和牙髓成纤维细胞对大多数测试物质的改变更为敏感。
It was the purpose of this investigation to determine the cytotoxic effects (ED50 concentrations) of 35 monomers or additives identified in commercial dental resin composites. Monolayers of permanent 3T3 cells and three primary human fibroblast types derived from oral tissues (gingiva, pulp, and periodontal ligament) were used as test systems. All substances were tested in concentrations ranging from 0.01 to 5.0 mM. In general, ED50 values varied from 0.06 to > 5 mM. Within the groups of co(monomers), initiators, and cointiators, severe (e.g., Bis-GMA, UDMA, DMBZ, and DMDTA) or moderate (HEMA, BEMA, CQ, DMPT, and DMAPE) cytotoxic effects could be evaluated. Within the group of reaction/decomposition products, only moderate or slight effects were found (ED50: 0.7 to > 5 mM). The inhibitor BHT, the contaminant TPSb, and the photostabilizer HMBP, however, were highly cytotoxic in all cell cultures. In addition, the ED50 values of DBPO and HMBP significantly varied (0.43-3.8 mM, respectively, and 0.44-3.07 mM) with the applied cell culture. Our comprehensive screening shows that for several of the highly cytotoxic composite components, less cytotoxic alternatives are available. Furthermore, there was no cell type identified which was consistently less or more sensitive to the toxic effects of the tested compounds than the others. Primary human periodontal ligament and pulp fibroblasts, however, were found to be more sensitive than 3T3 and gingival fibroblasts to alterations from most tested substances.