Polymerization Amplified Detection for Nanoparticle-Based Biosensing
Polymerization Amplified Detection for Nanoparticle-Based Biosensing
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DOI：
10.1021/nl502840h
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发表时间：
2014-11-01
期刊：
影响因子：
10.8
通讯作者：
Stevens, Molly M.
中科院分区：
文献类型：
--
作者：
Gormley, Adam J.;Chapman, Robert;Stevens, Molly M.
有效的信号放大过程是设计用于生物分子检测的灵敏测定的关键。在这里，我们描述了一种新的检测平台，该平台利用聚合反应和纳米颗粒的聚集来放大信号。在我们的设计中，建立了一个级联，其中酶或金属离子产生的自由基聚合形成聚合物，可以将多个金纳米颗粒（AuNP）缠结成聚集体，从而导致可见的颜色变化。引发聚集需要小于0.05%的单体至聚合物转化率，从而提供对自由基生成物质的高灵敏度。该测定对辣根过氧化物酶、过氧化氢酶和十亿分之几浓度的铁和铜具有良好的灵敏度。掺入耗氧酶葡萄糖氧化酶（GOx），使该测定能够在环境温度下在露天条件下进行。我们预计，这样的设计将提供一个有用的平台，通过聚合为基础的扩增范围广泛的生物分子的灵敏检测。
Efficient signal amplification processes are key to the design of sensitive assays for biomolecule detection. Here, we describe a new assay platform that takes advantage of both polymerization reactions and the aggregation of nanoparticles to amplify signal. In our design, a cascade is set up in which radicals generated by either enzymes or metal ions are polymerized to form polymers that can entangle multiple gold nanoparticles (AuNPs) into aggregates, resulting in a visible color change. Less than 0.05% monomer-to-polymer conversion is required to initiate aggregation, providing high sensitivity toward the radical generating species. Good sensitivity of this assay toward horseradish peroxidase, catalase, and parts per billion concentrations of iron and copper is shown. Incorporation of the oxygen-consuming enzyme glucose oxidase (GOx), enables this assay to be performed in open air conditions at ambient temperature. We anticipate that such a design will provide a useful platform for sensitive detection of a broad range of biomolecules through polymerization-based amplification.