4D electron microscopy of T cell activation.
4D electron microscopy of T cell activation.
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T 细胞激活的 4D 电子显微镜。
DOI：
10.1073/pnas.1914078116
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发表时间：
2019
影响因子：
11.1
通讯作者：
Heath,JamesR
中科院分区：
文献类型：
--
作者：
Lu,Yue;Yoo,Byung-Kuk;Ng,AlphonsusHC;Kim,Jungwoo;Yeom,Sinchul;Tang,Jau;Lin,MiloM;Zewail,AhmedH;Heath,JamesR
可以通过抗原特异性或非特异性方案可控地刺激 T 细胞。 T 细胞激活的伴随功能特征包括细胞骨架重组、细胞大小增加和细胞因子分泌。光子诱导近场电子显微镜 (PINEM) 用于对 T 细胞表面的瞬逝电场进行成像和量化，作为各种刺激条件的函数。虽然 PINEM 信号强度随与 T 细胞功能激活相关的多种生物物理变化而变化，但它主要与 T 细胞受体的抗原结合密切相关，即使在不会导致功能性 T 细胞激活的条件下也是如此。 PINEM 图像分析表明，刺激诱导的 T 细胞表面结构重组，尤其是在几百纳米的长度尺度上，是这些 PINEM 信号变化的主要贡献者。这些实验表明 PINEM 可以提供纳米级细胞表面结构的灵敏的无标记探针。
T cells can be controllably stimulated through antigen-specific or nonspecific protocols. Accompanying functional hallmarks of T cell activation can include cytoskeletal reorganization, cell size increase, and cytokine secretion. Photon-induced near-field electron microscopy (PINEM) is used to image and quantify evanescent electric fields at the surface of T cells as a function of various stimulation conditions. While PINEM signal strength scales with multiple of the biophysical changes associated with T cell functional activation, it mostly strongly correlates with antigen-engagement of the T cell receptors, even under conditions that do not lead to functional T cell activation. PINEM image analysis suggests that a stimulation-induced reorganization of T cell surface structure, especially over length scales of a few hundred nanometers, is the dominant contributor to these PINEM signal changes. These experiments reveal that PINEM can provide a sensitive label-free probe of nanoscale cellular surface structures.