Monitoring of phosphatase and kinase activity using 31P NMR spectroscopy
Monitoring of phosphatase and kinase activity using 31P NMR spectroscopy
复制标题
DOI：
10.1039/d3nj00642e
复制
发表时间：
2023-08-09
影响因子：
3.3
通讯作者：
Zhao，Yufen
中科院分区：
文献类型：
--
作者：
Guo，Xiaofan;Han，Bowen;Zhao，Yufen
传统上，酶测定需要添加标记或外源补充源。磷 31 核磁共振 (31P NMR) 光谱已被证明是一种强大且可靠的工具，但它很少用于评估酶活性。在此，我们提供了一种通用方法，通过利用底物和产物之间明显差异的 31P NMR 位移来直接表征磷酸酶和激酶活性，而不需要标签或额外的试剂。还介绍了该方法在抑制剂鉴定中的实用性。
Traditionally, enzymatic assays require the addition of labels or exogenous supplementation sources. Phosphorus-31 nuclear magnetic resonance (31P NMR) spectroscopy has proven to be a powerful and reliable tool, but it is scarcely employed to evaluate enzymatic activity. Herein, we provide a versatile method to directly characterize phosphatase and kinase activities by exploiting the apparently differential 31P NMR shifts between substrates and products, without requiring tags or additional reagents. The utility of this method for inhibitor identification is also presented.