Whole-brain connectivity to the bed nucleus of the stria terminalis calretinin-expressing interneurons in male mice
Whole-brain connectivity to the bed nucleus of the stria terminalis calretinin-expressing interneurons in male mice
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DOI：
10.1111/ejn.16068
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发表时间：
2023-07-15
影响因子：
3.4
通讯作者：
Wang，Lu
中科院分区：
文献类型：
--
作者：
Zhu，Ke-Wei;Tao，Gui-Jin;Wang，Lu
终纹床核（BNST）是一个富含神经肽的大脑区域，可以调节各种情绪行为和状态，包括压力，焦虑，奖励和社会互动。BNST由不同的亚区和神经元合奏，然而，由于BNST神经元内的高分子异质性，BNST调节不同的情绪行为的机制在很大程度上仍然不清楚。先前的研究已经确定了BNST钙视网膜蛋白（CR）表达神经元，缺乏神经肽。在这里，采用基于病毒的细胞类型特异性逆行和顺行追踪系统，我们绘制了雄性小鼠BNST CR表达神经元的全脑单突触输入和轴突投射。我们发现，BNST CR表达神经元主要从杏仁核过渡区、中央杏仁核和海马接受输入，并适度从内侧视前区、基底外侧杏仁核、室旁丘脑和外侧下丘脑接受输入。在BNST内，大量的输入神经元主要位于卵圆和束间亚区。此外，在BNST内观察到许多表达BNST CR的神经元终扣，但在其他脑区域中未观察到，因此表明这些神经元是一种中间神经元。这些结果将有助于进一步阐明BNST复杂而独特的功能背后的神经回路。
The bed nucleus of the stria terminalis (BNST) is a neuropeptide‐enriched brain region that modulates a wide variety of emotional behaviours and states, including stress, anxiety, reward and social interaction. The BNST consists of diverse subregions and neuronal ensembles; however, because of the high molecular heterogeneity within BNST neurons, the mechanisms through which the BNST regulates distinct emotional behaviours remain largely unclear. Prior studies have identified BNST calretinin (CR)‐expressing neurons, which lack neuropeptides. Here, employing virus‐based cell‐type‐specific retrograde and anterograde tracing systems, we mapped the whole‐brain monosynaptic inputs and axonal projections of BNST CR‐expressing neurons in male mice. We found that BNST CR‐expressing neurons received inputs mainly from the amygdalopiriform transition area, central amygdala and hippocampus and moderately from the medial preoptic area, basolateral amygdala, paraventricular thalamus and lateral hypothalamus. Within the BNST, plenty of input neurons were primarily located in the oval and interfascicular subregions. Furthermore, numerous BNST CR‐expressing neuronal boutons were observed within the BNST but not in other brain regions, thus suggesting that these neurons are a type of interneuron. These results will help further elucidate the neuronal circuits underlying the elaborate and distinct functions of the BNST.