Additively manufactured carbon/black-integrated polylactic acid 3Dprintedsensor for simultaneous quantification of uric acid and zinc in sweat
Additively manufactured carbon/black-integrated polylactic acid 3Dprintedsensor for simultaneous quantification of uric acid and zinc in sweat
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DOI：
10.1007/s00604-021-05007-5
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发表时间：
2021-11-01
影响因子：
5.7
通讯作者：
Angnes, Lucio
中科院分区：
文献类型：
--
作者：
Ataide, Vanessa N.;Rocha, Diego P.;Angnes, Lucio
首次描述了使用电化学处理的3D打印工作电极同时定量汗液中Zn 2+和尿酸（UA）的电化学方法的开发。汗液分析可以提供关于代谢物的重要信息，这些代谢物是生物过程的有价值的指标。在碱性介质中对其表面进行电化学处理后，3D打印电极的性能得到了改善。该处理促进PLA去除（绝缘层）并暴露炭黑（CB）导电部位。通过调节pH值和方波阳极溶出伏安法的参数，优化了方法的条件。在优化条件下使用经处理的表面，Zn 2+和UA的峰分别在约-1.1 V和+0.45 V（相对于CB/PLA伪参比）处明确定义。标准曲线显示线性范围为1至70 μ g L-1和1至70 μ mol L-1的Zn 2+和UA，分别。Zn ~（2+）和UA的相对标准偏差分别为4.8%（n = 10，30 μ g L ~（-1））和6.1%（n = 10，30 μ mol L ~（-1））。Zn ~（2+）和UA的检出限分别为0.10 μ g L ~（-1）和0.28 μ mol L ~（-1）。这两个物种同时测定在真实的汗液样品中，和实现的回收率为95和106%之间的Zn 2+和82和108%的UA。
For the first time the development of an electrochemical method for simultaneous quantification of Zn2+ and uric acid (UA) in sweat is described using an electrochemically treated 3D-printed working electrode. Sweat analysis can provide important information about metabolites that are valuable indicators of biological processes. Improved performance of the 3D-printed electrode was achieved after electrochemical treatment of its surface in an alkaline medium. This treatment promotes the PLA removal (insulating layer) and exposes carbon black (CB) conductive sites. The pH and the square-wave anodic stripping voltammetry technique were carefully adjusted to optimize the method. The peaks for Zn2+ and UA were well-defined at around - 1.1 V and + 0.45 V (vs. CB/PLA pseudo-reference), respectively, using the treated surface under optimized conditions. The calibration curve showed a linear range of 1 to 70 mu g L-1 and 1 to 70 mu mol L-1 for Zn2+ and UA, respectively. Relative standard deviation values were estimated as 4.8% (n = 10, 30 mu g L-1) and 6.1% (n = 10, 30 mu mol L-1) for Zn2+ and UA, respectively. The detection limits for Zn2+ and UA were 0.10 mu g L-1 and 0.28 mu mol L-1, respectively. Both species were determined simultaneously in real sweat samples, and the achieved recovery percentages were between 95 and 106% for Zn2+ and 82 and 108% for UA.