Ion-exchange high-performance liquid chromatography analysis of oligodeoxyribonucleotide phosphorothioates.
Ion-exchange high-performance liquid chromatography analysis of oligodeoxyribonucleotide phosphorothioates.
复制标题
寡脱氧核糖核苷酸硫代磷酸酯的离子交换高效液相色谱分析。
DOI：
10.1016/0003-2697(92)90476-n
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发表时间：
1992
影响因子：
2.9
通讯作者：
Agrawal,S
中科院分区：
文献类型：
--
作者：
Metelev,V;Agrawal,S
含硫代寡核苷酸的骨架已被用来调节病毒和细胞基因的表达。在组织培养中进行的研究表明，硫代寡核苷酸作为抗病毒药物的应用前景看好，目前正在研究开发这些寡核苷酸类似物作为化疗药物。为了分析和纯化寡核苷酸类似物，建立了采用弱阴离子交换柱的高效液相色谱分离方法。发现硫代寡核苷酸的分离是长度依赖的。
Oligodeoxynucleotide-containing phosphorothioate backbones have been used to regulate viral as well as cellular gene expression. The studies carried out in tissue culture have shown promising results on the use of oligonucleotide phosphorothioates as antiviral agents and, at present, study is underway to develop these oligonucleotide analogues as chemotherapeutic agents. To analyze and purify oligonucleotide analogues, high-performance liquid chromatography using weak anion exchange column has been described. The separation of oligonucleotide phosphorothioate is found to be length dependent.