Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation
Single cell analysis of gene expression patterns during carbon starvation in Bacillus subtilis reveals large phenotypic variation
复制标题
DOI：
10.1111/j.1462-2920.2012.02892.x
复制
发表时间：
2012-12-01
影响因子：
5.1
通讯作者：
Kuipers, Oscar P.
中科院分区：
文献类型：
--
作者：
de Jong, Imke G.;Veening, Jan-Willem;Kuipers, Oscar P.
细胞如何动态地响应波动的环境条件取决于基础遗传电路的结构和噪声。大多数工作表征压力途径的模式细菌枯草芽孢杆菌进行了大量的文化，使用合奏测定。然而，研究单细胞对应激的反应是重要的，因为噪声可能产生显著的表型异质性。在这里，我们研究了碳源饥饿的应激反应，并比较了群体和单细胞数据。采用自上而下的方法，我们研究了B的各种胁迫相关基因的转录动态。枯草芽孢杆菌对碳源饥饿和增加的细胞密度的反应。我们的数据表明，大多数测试的基因调控网络响应高度异质性饥饿和细胞表现出很大程度的基因表达的变化。B内部高度动态的多样化程度。枯草杆菌群体在变化的环境下的变化反映了在单细胞水平上研究细胞的必要性。
How cells dynamically respond to fluctuating environmental conditions depends on the architecture and noise of the underlying genetic circuits. Most work characterizing stress pathways in the model bacterium Bacillus subtilis has been performed on bulk cultures using ensemble assays. However, investigating the single cell response to stress is important since noise might generate significant phenotypic heterogeneity. Here, we study the stress response to carbon source starvation and compare both population and single cell data. Using a top-down approach, we investigate the transcriptional dynamics of various stress-related genes of B.?subtilis in response to carbon source starvation and to increased cell density. Our data reveal that most of the tested gene-regulatory networks respond highly heterogeneously to starvation and cells show a large degree of variation in gene expression. The level of highly dynamic diversification within B.?subtilis populations under changing environments reflects the necessity to study cells at the single cell level.