High-performance liquid chromatographic studies on non-mercapt in equilibrium with mercapt conversion of human serum albumin. II.
High-performance liquid chromatographic studies on non-mercapt in equilibrium with mercapt conversion of human serum albumin. II.
复制标题
人血清白蛋白中非硫醇与硫醇转化平衡的高效液相色谱研究。
DOI：
--
复制
发表时间：
1985
期刊：
影响因子：
--
通讯作者：
E. Tomita
中科院分区：
文献类型：
--
作者：
M. Sogami;S. Era;S. Nagaoka;K. Kuwata;K. Kida;J. Shigemi;K. Miura;E. Suzuki;Y. Muto;E. Tomita
在GS-520色谱柱上，以0.03 M磷酸钠缓冲液-0.15 M硫酸钠（pH 6.87）对人血清白蛋白（HSA）进行高效液相色谱分析，发现主成分为人巯基白蛋白（HMA），二级和三级成分为非巯基白蛋白（HNA）。通过HPLC分析，再次证实血液透析时HSA的非巯基----巯基转化和慢性肾衰竭血液透析后的巯基----非巯基转化，表明HMA是含硫氨基酸的共价载体蛋白。不同肝脏疾病患者的HMA含量明显低于健康成年男性。
High-performance liquid chromatographic (HPLC) analysis of human serum albumin (HSA) on a GS-520 column with 0.03 M sodium phosphate buffer-0.15 M sodium sulphate (pH 6.87) showed three peaks, the principal component corresponding to human mercaptalbumin (HMA) and the secondary and tertiary components to nonmercaptalbumin (HNA). Using HPLC analysis, the nonmercapt----mercapt conversion of HSA during haemodialysis and the mercapt----nonmercapt conversion after haemodialysis in chronic renal failure were re-confirmed, indicating that HMA is a covalent carrier protein for sulphur-containing amino acids. Fractions of HMA in various liver diseases were significantly lower than those of healthy male adults.