SIMPLE ENZYMIC METHOD FOR ISOLATION OF DNA FROM DIVERSE BACTERIA
SIMPLE ENZYMIC METHOD FOR ISOLATION OF DNA FROM DIVERSE BACTERIA
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DOI：
10.1016/0167-7012(89)90001-8
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发表时间：
1989-09-01
影响因子：
2.2
通讯作者：
MCFADDEN, JJ
中科院分区：
文献类型：
--
作者：
VISUVANATHAN, S;MOSS, MT;MCFADDEN, JJ
本文描述了一种从包括分枝杆菌在内的多种细菌中提取高分子量DNA的酶法。该方法简单、快速，需要很少的操作，并且适合于从少至约1000 μ g的DNA中提取定量产率的DNA。3倍。107个细菌细胞所产生的DNA具有良好的质量，并且适合于分子生物学操作。
An enzymic method for the extraction of high molecular weight DNA from a wide range of diverse bacteria, including mycobacteria, is described. The method is simple, rapid, requires few manipulations and is suitable for extracting quantitative yields of DNA from as few as .apprxeq. 3 .times. 107 bacterial cells. The DNA produced is of good quality and is suitable for molecular biological manipulations.