Vimentin and keratin intermediate filament systems in cultured PtK2 epithelial cells are interrelated.
Vimentin and keratin intermediate filament systems in cultured PtK2 epithelial cells are interrelated.
复制标题
培养的 PtK2 上皮细胞中的波形蛋白和角蛋白中间丝系统是相互关联的。
作者：
M. Klymkowsky
某些培养的上皮细胞含有单独的波形蛋白和角蛋白型中间纤维网络。向PtK2培养的上皮细胞内注射针对波形蛋白或角蛋白丝的单克隆抗体，特异性地破坏了这两种丝的组织。当将这两种抗体注射到含有波形蛋白或角蛋白丝的细胞中，缺乏抗体识别的特定纤维类型时，这两种抗体都没有任何作用。这些实验表明角蛋白和波形蛋白丝网络在某种程度上相互关联。
Certain cultured epithelial cells contain separate vimentin and keratin‐type intermediate filament networks. The intracellular injection of monoclonal antibodies directed against either vimentin or keratin filaments into PtK2 cultured epithelial cells specifically disrupted the organization of both filament types. Neither antibody had any effect when injected into cells which, while containing vimentin or keratin filaments, lacked the specific filament type which that antibody recognized. These experiments suggest that keratin and vimentin filament networks are associated in some way with one another.