A simple artificial urine for the growth of urinary pathogens
A simple artificial urine for the growth of urinary pathogens
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DOI：
10.1046/j.1472-765x.1997.00378.x
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发表时间：
1997-03-01
影响因子：
2.4
通讯作者：
Keevil, CW
中科院分区：
文献类型：
--
作者：
Brooks, T;Keevil, CW
已经开发了一种简单的人工尿培养基（AUM），其提供与人尿中发现的条件类似的条件。用琼脂固化的AUM能够回收广泛的尿素酶阳性和阴性泌尿系病原体。液体AUM在浓度高达10（8）cfu ml（-1）时支持生长，与正常尿液中发现的一样。在连续培养中，可重复的稳态生长也发生在许多代中。AUM能够形成类似于自然尿路感染中发现的晶体和结壳。该培养基是正常尿液的合适替代品，可用于模拟临床环境中泌尿系病原体生长和附着的广泛实验。
A simple artificial urine medium (AUM) has been developed which provides conditions similar to that found in human urine. AUM solidified with agar enabled the recovery of a wide range of urease-positive and -negative urinary pathogens. Liquid AUM supported growth at concentrations of up to 10(8) cfu ml(-1), as found in normal urine. Reproducible, steady-state growth also occurred over many generations in continuous culture. AUM was capable of forming crystals and encrustations resembling those found in natural urinary tract infections. The medium is a suitable replacement for normal urine for use in a wide range of experiments modelling the growth and attachment of urinary pathogens in the clinical environment.