Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds
Enhancing the proliferation of MC3T3-E1 cells on casein phosphopeptide-biofunctionalized 3D reduced-graphene oxide/polypyrrole scaffolds
复制标题
增强酪蛋白磷酸肽生物功能化 3D 还原氧化石墨烯/聚吡咯支架上 MC3T3-E1 细胞的增殖
DOI：
10.1039/c7ra02146a
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发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Liu, Bin
中科院分区：
文献类型：
--
作者：
Jie, Weibo;Song, Fuxiang;Liu, Bin
在骨组织工程中，三维宏观支架作为细胞增殖、分化和组织形成的基质起着不可或缺的作用，而细胞增殖、分化和组织形成需要生物活性表面。以前，我们成功地制备了三维大还原氧化石墨烯/聚吡咯(3DrGO/PPY)支架；本研究以酪蛋白磷酸肽(CPP)为生物活性分子，通过一种简单、低成本的静电自组装方法制备了3DrGO/PPY/CPP复合支架，并探索了该复合支架在骨组织工程中的应用。结果表明，CPP成功地对支架骨架进行了改性，制备的3DrGO/PPY/CPP支架具有良好的亲水性和吸水性，明显优于3DrGO/PPY支架。在仿生矿化实验中，CPP修饰的基质，尤其是3DrGO/PPY/CPP20，在模拟体液中仅在浸泡第1天就能促进羟基磷灰石的快速形成。与MC3T3-E1细胞共培养时，3d rGO/PPY/CPP20组细胞处于较高的增殖状态，是对照组的5.07倍，优于3d rGO/PPY/CPP10组(3.88倍)和3d rGO/PPY组(2.07倍)。3DRGO/PPY/CPP20复合支架良好的成骨性能归功于CPP良好的生物学性能和独特的具有高比表面积的三维宏观结构。我们的研究结果表明，3D rGO/PPY/CPP20可以被认为是一种有吸引力的未来骨愈合和再生的支架。
In bone tissue engineering, the 3D macro-scaffold plays an indispensable role as a matrix for cell proliferation, differentiation and tissue formation, for which a bioactive surface is required. Previously, we successfully prepared the 3D macro-reduced graphene oxide/polypyrrole (3D rGO/PPY) scaffold; in the present study, by using casein phosphopeptide (CPP) as a bioactive molecule, we have developed a 3D rGO/PPY/CPP composite scaffold through a simple, but low-cost electrostatic self-assembly method and have explored the application of the composite scaffold in bone tissue engineering. The results have indicated that the CPP successfully modified the backbone of the scaffold, and demonstrated that the developed 3D rGO/PPY/CPP scaffold has excellent hydrophilic behavior and water uptake performance, much better than that of 3D rGO/PPY. In the biomimetic mineralization experiment, the CPP-modified matrix, particularly 3D rGO/PPY/CPP20, could promote the rapid formation of hydroxyapatite in simulated body fluid solution on just the 1st soaking day. On co-culturing with the MC3T3-E1 cells, the 3D rGO/PPY/CPP20 kept the cells at a higher proliferation state of 5.07 times that of the control group, superior to that of the 3D rGO/PPY/CPP10 (3.88 times) and the 3D rGO/PPY group (2.07 times). The excellent osteoblastic performance of the 3D rGO/PPY/CPP20 composite scaffold can be attributed to the good biological properties of CPP, and the unique 3D macro-structure with a high specific surface area. Our findings suggest that the 3D rGO/PPY/CPP20 can be considered as an attractive scaffold for bone healing and regeneration in the future.