Mimicking Chemotactic Cell Migration with DNA Programmable Synthetic Vesicles
Mimicking Chemotactic Cell Migration with DNA Programmable Synthetic Vesicles
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DOI：
10.1021/acs.nanolett.9b04428
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发表时间：
2019-12-01
期刊：
影响因子：
10.8
通讯作者：
Choi, Jong Hyun
中科院分区：
文献类型：
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作者：
Pan, Jing;Du, Yancheng;Choi, Jong Hyun
趋化细胞运动在许多生物学功能中起着关键作用，如免疫反应和胚胎发生。构建合成细胞模拟系统，如动态原始细胞，同样需要响应环境刺激并执行程序化运动行为的分子机制。虽然提出了各种分子成分，以实现不同的功能，在合成的原始细胞，表面上的趋化运动迄今尚未报道。在这里，我们展示了合成脂质囊泡的定向运动能力，它们能够通过编程DNA成分相互追逐。我们证明，“跟随”囊泡识别和迁移沿着移动轨迹的“铅”囊泡与增强的速度，从而模仿自然的趋化性细胞迁移。这项工作为构建具有复杂功能的合成原始细胞提供了新的可能性，如程序化形态发生和合作运动。凭借庞大的动态DNA组件库，我们设想该平台将使基础科学的新发现和生物技术的新应用成为可能。
Chemotactic cell motility plays a critical role in many biological functions, such as immune response and embryogenesis. Constructing synthetic cell-mimicking systems, such as a dynamic protocell, likewise requires molecular mechanisms that respond to environmental stimuli and execute programmed motility behaviors. Although various molecular components were proposed to achieve diverse functions in synthetic protocells, chemotactic motility on surfaces has not been reported thus far. Here we show directional motility in synthetic lipid vesicles capable of chasing each other by programming DNA components. We demonstrate that the "follow" vesicle recognizes and migrates along the moving trajectory of the "lead" vesicle with an enhanced speed, thus mimicking natural chemotaxis in cell migration. This work provides new possibilities for building synthetic protocells with complex functions such as programmed morphogenesis and cooperative motion. With the vast library of dynamic DNA components, we envision that this platform will enable new discoveries in fundamental sciences and novel applications in biotechnology.