DIFFERENTIAL CELLULAR-LOCALIZATION OF 3 SPLICE VARIANTS OF THE MGLUR1 METABOTROPIC GLUTAMATE-RECEPTOR IN RAT CEREBELLUM
DIFFERENTIAL CELLULAR-LOCALIZATION OF 3 SPLICE VARIANTS OF THE MGLUR1 METABOTROPIC GLUTAMATE-RECEPTOR IN RAT CEREBELLUM
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DOI：
10.1097/00001756-199411000-00011
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发表时间：
1994-11-21
期刊：
影响因子：
1.7
通讯作者：
KNOPFEL, T
中科院分区：
文献类型：
--
作者：
GRANDES, P;MATEOS, JM;KNOPFEL, T
针对大鼠mGluR1代谢性谷氨酸受体剪接变体a、b和c的c端肽产生抗体。分别与mGluR1a、mGluR1b和mGluR1c特异性反应的亲和纯化抗体用于研究这些受体在大鼠小脑中的细胞定位。mGluR1a抗体强烈标记浦肯野细胞的细胞体、部分树突树和许多小点状元件，使人想起树突棘。还标记了一些星状细胞、篮状细胞、高尔基细胞和卢加罗细胞。颗粒细胞无染色。mGluR1b抗体强烈标记浦肯野细胞体及其树突，其水平在蚓部或半球的同一小叶内变化。在星状细胞、篮状细胞、高尔基体细胞和颗粒细胞中未观察到明显的标记，而偶尔有一小部分篮状细胞和小脑肾小球出现中度免疫反应。mGluR1c抗体强烈标记浦肯野细胞的细胞体和粗主树突，但不标记树突棘。免疫阴性的浦肯野细胞与强标记的浦肯野细胞混杂在4-10小叶中，而在1、2和3小叶中未检测到染色的浦肯野细胞。mGluR1c抗体还标记了星状细胞、篮状细胞、部分高尔基细胞和部分卢加罗细胞以及颗粒细胞。
ANTIBODIES were raised against C-terminal peptides of the splice variants a, b and c of the rat mGluR1 metabotropic glutamate receptor. Affinity purified antibodies each specifically reacting with mGluR1a, mGluR1b and mGluR1c were used to study the cellular localization of these receptors in rat cerebellum. The mGluR1a antibody strongly labelled Purkinje cells at their cells bodies, portions of their dendritic trees and numerous small punctate elements reminiscent of dendritic spines. Also labelled were some stellate, basket, Golgi and Lugaro cells. Granule cells were devoid of staining. The mGluR1b antibody strongly labelled Purkinje cell bodies and their dendrites at levels which varied within the same lobule of the vermis or the hemispheres. No significant labelling was observed at stellate, basket, Golgi and granule cells, while occasionally a fraction of basket cells and cerebellar glomeruli was moderately immunoreactive. The mGluR1c antibody strongly labelled cell bodies and thick principal dendrites of Purkinje cells but not dendritic spines. Immunonegative Purkinje cells were intermingled with strongly labelled ones in lobules 4-10, while in lobules 1, 2 and 3, no stained Purkinje cells were detected. The mGluR1c antibody also labelled stellate, basket, some Golgi and some Lugaro cells as well as granule cells.