THE MOTILE RESPONSE OF ALVEOLAR MACROPHAGES - AN EXPERIMENTAL-STUDY USING SINGLE-CELL AND CELL-POPULATION APPROACHES
THE MOTILE RESPONSE OF ALVEOLAR MACROPHAGES - AN EXPERIMENTAL-STUDY USING SINGLE-CELL AND CELL-POPULATION APPROACHES
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DOI：
10.1164/ajrccm/139.2.320
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发表时间：
1989-02-01
期刊：
影响因子：
--
通讯作者：
DANIELE, RP
中科院分区：
文献类型：
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作者：
GLASGOW, JE;FARRELL, BE;DANIELE, RP
在这份报告中，我们研究了单个细胞运动的随机游走模型在预测琼脂糖下肺泡巨噬细胞群体运动行为中的适用性。在不存在趋化性或趋化动力学梯度的情况下，细胞群的迁移可以通过随机运动系数μ来表征，其类似于粒子扩散系数。随机游走理论将后一个系数与粒子速度和碰撞时间（相当于粒子方向变化之间的时间）联系起来。通过类比，根据细胞迁移的类似随机游走理论，μ对于一个细胞群体来说，它是单个细胞的速度和持续时间的函数（方向的变化由细胞的行为过程而不是由碰撞决定）。为了测试该模型，在存在或不存在均匀浓度的趋化三肽甲酰-正亮氨酰亮氨酰苯丙氨酸（FNLLP）的情况下孵育正常豚鼠肺泡巨噬细胞，以引起不同水平的运动活性。μ。从通过在2、3或4天后固定和染色培养物获得的细胞群体密度分布计算。在平行实验中，单个细胞的速度和持续时间从1小时，延时视频显微镜记录测量。μ的值。从单细胞测量计算的与刺激随机迁移的群体研究（在10-7至10-11 M FNLLP下）的结果良好一致，但在完全不存在刺激物的情况下不一致。总体而言，这些结果支持单个细胞迁移的随机游走模型对随机迁移的肺泡巨噬细胞群体的适用性。
In this report, we studied the applicability of a random walk model of individual cell motility in predicting the motile behavior of alveolar macrophage populations under agarose. The migration of a population of cells in the absence of a chemotactic or chemokinetic gradient can be characterized by the random motility coefficient, .mu., which is analogous to a particle diffusion coefficient. Random walk theory relates this latter coefficient to particle speed and collision time (equivalent to the time between changes in particles direction). By analogy, according to a similar random walk therory for cell migration, .mu. for a cell population is a function of the speed and persistence time (with direction changes governed by cell behavioral processess rather than by collisions) of individual cells. To test the model, normal guinea pig alveolar macrophages were incubated in the presence or absence of uniform concentrations of the chemotatic tripeptide formy-norleucyl leucyl phenylalanine (FNLLP) to elicit different levels of motile activity. .mu. was calculated from cell population density profiles obtained by fixing and staining cultures after 2, 3, or 4 days. In parallel experiments, individual cell speeds and persistence times were measured from 1-h, time-lapse video microscopy recordings. The value of .mu. calculated from single-cell measurements was in good agreement with that from population studies for stimulated random migration (at 10-7 to 10-11 M FNLLP), but not in athe absence of stimulant. Overall, these results support the applicability of the random walk model of individual cell migration to randomly migrating alveolar macrophage populations.