Single-nucleotide polymorphisms in PtoCesA7 and their association with growth and wood properties in Populus tomentosa
Single-nucleotide polymorphisms in PtoCesA7 and their association with growth and wood properties in Populus tomentosa
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PtoCesA7 的单核苷酸多态性及其与毛白杨生长和木材特性的关系
DOI：
10.1007/s00438-014-0824-6
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发表时间：
2014-06-01
影响因子：
3.1
通讯作者：
Zhang, Deqiang
中科院分区：
文献类型：
--
作者：
Tian, Jiaxing;Chang, Mengqi;Zhang, Deqiang
纤维素合成酶（CesA）基因编码合成纤维素的酶;因此，CesA在植物发育中起核心作用，并影响木材的产量和质量，这是植物生物质工业应用的基本特性。为了有效地控制木材的生物合成，提高木材质量，我们需要更好地了解天然变异的CesAs。关联研究已成为鉴定数量性状变异的有力工具。在这里，我们使用了一个候选基因为基础的关联作图方法，以确定PtoCesA 7等位基因变异与毛白杨的生长和木材质量性状。我们分离了全长PtoCesA 7 cDNA，并观察到高PtoCesA 7在木质部中的表达，与CesA 7的木质部特异性表达一致。从自然分布的毛白杨中提取的PtoCesA 7的核苷酸多样性和连锁不平衡（LD）分析表明，PtoCesA 7具有高的核苷酸多样性（π（T）= 0.0091）和低的LD（r（2）≥ 0.1，在800 bp以内）。通过关联分析，我们发现了7个单核苷酸多态性（SNPs）（错误发现率Q < 0.10）和12个单倍型（Q < 0.10）与生长和木材性质相关，解释表型方差的3.62%-10.59%。我们还验证了9个10个显着的标记性状协会在至少三个较小的子集（气候区）或在连锁映射人口之一。因此，我们的研究确定了与生长和木材质量性状相关的功能性PtoCesA 7等位基因变体，为影响木材质量和数量的基因提供了新的见解。从应用的角度来看，本研究揭示的SNPs在分子标记辅助育种中具有潜在的应用价值。
Cellulose synthase (CesA) genes encode the enzymes that synthesize cellulose; therefore, CesAs play central roles in plant development and affect the yield and quality of wood, essential properties for industrial applications of plant biomass. To effectively manipulate wood biosynthesis in trees and improve wood quality, we thus require a better understanding of the natural variation in CesAs. Association studies have emerged as a powerful tool for identification of variation associated with quantitative traits. Here, we used a candidate gene-based association mapping approach to identify PtoCesA7 allelic variants that associate with growth and wood quality traits in Populus tomentosa. We isolated a full-length PtoCesA7 cDNA and observed high PtoCesA7 expression in xylem, consistent with the xylem-specific expression of CesA7. Nucleotide diversity and linkage disequilibrium (LD) in PtoCesA7, sampled from the P. tomentosa natural distribution, revealed that PtoCesA7 harbors high nucleotide diversity (π(T) = 0.0091) and low LD (r(2) ≥ 0.1, within 800 bp). By association analysis, we identified seven single-nucleotide polymorphisms (SNPs) (false discovery rate Q < 0.10) and 12 haplotypes (Q < 0.10) that associated with growth and wood properties, explaining 3.62-10.59 % of the phenotypic variance. We also validated 9 of the 10 significant marker-trait associations in at least one of three smaller subsets (climatic regions) or in a linkage-mapping population. Thus, our study identified functional PtoCesA7 allelic variants associated with growth and wood quality traits, giving new insights into genes affecting wood quality and quantity. From an applied perspective, the SNPs revealed in this study have potential applications in marker-assisted breeding.