Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces
Autofocusing MALDI mass spectrometry imaging of tissue sections and 3D chemical topography of nonflat surfaces
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DOI：
10.1038/nmeth.4433
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发表时间：
2017-12-01
期刊：
影响因子：
48
通讯作者：
Spengler, Bernhard
中科院分区：
文献类型：
--
作者：
Kompauer, Mario;Heiles, Sven;Spengler, Bernhard
我们描述了一种大气压基质辅助激光解吸-电离质谱仪成像系统，它使用微米级的长距离激光三角测量来同时从3D表面获得地形和分子信息。我们研究了化合物在植物和寄生虫不规则3D表面上的地形分布，并以高横向分辨率对非平面组织切片进行成像，从而消除了与高度相关的信号伪影。
We describe an atmospheric pressure matrix-assisted laser desorption-ionization mass spectrometry imaging system that uses long-distance laser triangulation on a micrometer scale to simultaneously obtain topographic and molecular information from 3D surfaces. We studied the topographic distribution of compounds on irregular 3D surfaces of plants and parasites, and we imaged nonplanar tissue sections with high lateral resolution, thereby eliminating height-related signal artifacts.