Synthesis, functionalization and bioimaging applications of highly fluorescent carbon nanoparticles
Synthesis, functionalization and bioimaging applications of highly fluorescent carbon nanoparticles
复制标题
DOI：
10.1039/c0nr00735h
复制
发表时间：
2011-01-01
期刊：
影响因子：
6.7
通讯作者：
Pramanik, Panchanan
中科院分区：
文献类型：
--
作者：
Chandra, Sourov;Das, Pradip;Pramanik, Panchanan
以蔗糖和磷酸为原料，在100 W微波辐射3 min 40 s的条件下，一步合成了具有高荧光的结晶碳纳米颗粒（CNPs）。该方法非常简单、快速和经济，因此可用于大规模应用。平均粒径为3 ~ 10 nm，在紫外光照射下发出明亮的绿色荧光。因此，该颗粒可用作生物成像以及药物递送的独特材料。为了进一步增加合成碳纳米颗粒的荧光性质，我们简单地通过使用不同的有机染料，如荧光素、罗丹明B和α-萘胺将它们官能化;对于用荧光素官能化的颗粒观察到最大荧光强度。非常有趣的是，注意到所有这些化合物在225 nm激发波长处显示最大荧光强度，并且对于任何激发波长，峰位置与CNP本身的位置完全相同，这与单独的前体（染料）完全不同。所有上述化合物，包括CNP，也已成功地引入健康人血细胞的红细胞富集部分，具有最小的细胞毒性。
Highly fluorescent crystalline carbon nanoparticles (CNPs) have been synthesized by one step microwave irradiation of sucrose with phosphoric acid at 100 W for 3 min 40 s. This method is very simple, rapid and economical and hence can be used for large scale applications. The average particle sizes are 3 to 10 nm and they emit bright green fluorescence under the irradiation of UV-light. Therefore, the particles can be used as a unique material for bioimaging as well as drug delivery. To further increase the fluorescence property of the synthetic carbon nanoparticles we simply functionalized them by using different organic dyes, such as fluorescein, rhodamine B and alpha-naphthylamine; the maximum fluorescence intensity was observed for the particles functionalized with fluorescein. It is very interesting to note that all of those compounds show maximum fluorescence intensity at 225 nm excitation wavelength and for any excitation wavelength the peak positions are exactly same the position as that of CNPs itself, which is completely different from the individual precursors (dyes). All of the above compounds, including CNPs, have also been successfully introduced into the erythrocyte enriched fraction of healthy human blood cells with minimum cytotoxicity.