Na,K-ATPase α and β subunit genes exhibit unique expression patterns during zebrafish embryogenesis
Na,K-ATPase α and β subunit genes exhibit unique expression patterns during zebrafish embryogenesis
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DOI：
10.1016/s0925-4773(02)00135-1
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发表时间：
2002-08-01
影响因子：
2.6
通讯作者：
Levenson, R
中科院分区：
文献类型：
--
作者：
Canfield, VA;Loppin, B;Levenson, R
我们使用原位杂交来分析斑马鱼胚胎发生过程中 Na,K-ATPase α 和 β 亚基基因的表达。最引人注目的发现是 14 个 Na,K-ATP 酶基因中的每一个都表现出独特的表达谱。所有α和β亚基基因都在神经系统中表达，尽管不同区域的表达模式差异很大。在外周组织中，五种α1样基因中的三种在前肾和粘液细胞中表达，一种在心脏中表达，一种主要在骨骼肌中表达。 α2 基因在大脑和心脏中表达，但在骨骼肌中最为突出，而两个 α3 基因的表达仅限于神经系统。在六个 β 亚基基因中，β1a 在晶状体、前肾和心脏中表达量最高，而 β1b 转录本在粘液细胞中丰富。这两个 beta2 样基因在神经系统中的表达存在差异。一种 beta3 基因仅在大脑中表达，而另一种则在骨骼肌中大量表达。基于这些表达模式，我们预测在不同组织中可能形成至少 14 个 α/β 亚基对。 (C) 2002 Elsevier Science Ireland Ltd. 保留所有权利。
We have used in situ hybridization to analyze Na,K-ATPase alpha and beta subunit gene expression during zebrafish embryogenesis. The most striking finding is that each of the 14 Na,K-ATPase genes exhibits a distinct expression profile. All alpha and beta subunit genes are expressed in the nervous system, although the pattern of expression in different regions varies dramatically. In peripheral tissues, three of the five alpha1-like genes are expressed in pronephros and mucous cells, one is expressed in heart, and one is predominant in skeletal muscle. The alpha2 gene is expressed in brain and heart but is most prominent in skeletal muscle, while the two alpha3 genes are restricted in their expression to the nervous system. Of the six beta subunit genes, beta1a is expressed at highest abundance in lens, pronephros, and heart, while beta1b transcripts are abundant in mucous cells. The two beta2-like genes are differentially expressed in the nervous system. One beta3 gene is expressed exclusively in brain while the other is abundantly expressed in skeletal muscle. Based on these expression patterns, we predict that at least 14 alpha/beta subunit pairs are likely to be formed in different tissues. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.