Cell Type Specific Representation of Vibro-tactile Stimuli in the Mouse Primary Somatosensory Cortex
Cell Type Specific Representation of Vibro-tactile Stimuli in the Mouse Primary Somatosensory Cortex
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DOI：
10.3389/fncir.2018.00109
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发表时间：
2018-12-20
影响因子：
3.5
通讯作者：
Ohki, Kenichi
中科院分区：
文献类型：
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作者：
Hayashi, Ayako;Yoshida, Takashi;Ohki, Kenichi
虽然在啮齿动物的初级躯体感觉皮层（S1）的桶区的胡须偏转的处理已被广泛研究，如何皮肤振动触觉刺激处理在啮齿动物的Si桶区以外的还没有得到充分的研究。特别地，尚未检查Si中遗传鉴定的抑制性细胞中的多个振动频率的细胞类型特异性表示。利用双光子钙离子成像技术，研究了雄性和雌性小鼠Si后肢区兴奋性和抑制性神经元对振动刺激的反应。兴奋性细胞对振动刺激表现出相对尖锐的选择性，而抑制性细胞表现出较小的选择性。不同偏好刺激的兴奋性和抑制性细胞以“盐和胡椒”的方式混合。此外，在具有相似刺激选择性的兴奋-抑制和兴奋-抑制细胞对中，噪声相关性往往特别强。这些结果表明，兴奋性细胞倾向于代表特定的刺激信息，并与类似调谐的抑制性细胞一起工作，作为一个功能连接的网络。
Although the processing of whisker deflections in the barrel area of the rodent primary somatosensory cortex (S1) has been studied extensively, how cutaneous vibro-tactile stimuli are processed in the rodent Si outside the barrel area has not been fully examined. Particularly, the cell-type specific representation of multiple vibration frequencies in genetically identified inhibitory cells in the Si has not been examined. Using two-photon calcium imaging, we examined the responses to vibration stimuli of excitatory and inhibitory neurons in the Si hind limb area of male and female mice. The excitatory cells showed relatively sharp selectivity to vibration stimuli, whereas the inhibitory cells exhibited less selectivity. The excitatory and inhibitory cells with different preferred stimuli were intermingled in a "salt and pepper" manner. Furthermore, the noise correlation tended to be especially strong in excitatory -inhibitory and inhibitory-inhibitory cell pairs that have similar stimulus selectivity. These results suggest that excitatory cells tend to represent specific stimulus information and work together with similarly tuned inhibitory cells as a functionally connected network.