Comparison by electrophoresis of proteins characteristic of the lateral line and skin of Xenopus laevis.
Comparison by electrophoresis of proteins characteristic of the lateral line and skin of Xenopus laevis.
复制标题
非洲爪蟾侧线和皮肤蛋白质特征的电泳比较。
DOI：
10.1016/0305-0491(84)90333-x
复制
发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Drescher,DG
中科院分区：
文献类型：
--
作者：
Drescher,DG
在实验中，旨在确定acousticolateralis标记蛋白，组分的侧线器官和皮肤的非洲爪蟾进行了分析，通过一维和二维聚丙烯酰胺凝胶电泳。约44K mol. wt（K= 1000道尔顿）和等电点pH 6.3，至少由两个组件组成，增强侧线神经肥大组织（含有毛细胞），并减少触觉器官和皮肤（缺乏毛细胞）。这种”神经瘤标志物蛋白”级分具有mol. wt接近肌动蛋白，但显示不同于肌动蛋白。其他两种主要蛋白质，摩尔。wts 16和28K在皮肤凝胶中存在，而在侧线组织凝胶中不存在。这些蛋白质被证明是由于两栖动物颗粒腺的分泌，并被指定为“阴性标记蛋白”。
In experiments aimed at determining acousticolateralis marker proteins, fractions of lateral-line organs and skin of Xenopus laevis were analyzed by one-and two-dimensional polyacrylamide-gel electrophoresis. A protein fraction of approximately 44K mol. wt (K= 1000 daltons) and isoelectric pH 6.3, consisting of at least two components, was enhanced in lateral-line neuromast tissue (containing hair cells) and was decreased in tactile organs and skin (lacking hair cells). This" neuromast-marker-protein" fraction had a mol. wt close to that of actin but was shown to be different from actin. Two other major proteins, at mol. wts 16 and 28K, were present in gels of skin and absent in gels of lateral-line tissue. These proteins were shown to be due to secretion of the amphibian granular glands and were designated" negative marker proteins".