Three-dimensional reconstruction of stained histological slices and 3D non-linear registration with in-vivo MRI for whole baboon brain
Three-dimensional reconstruction of stained histological slices and 3D non-linear registration with in-vivo MRI for whole baboon brain
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DOI：
10.1016/j.jneumeth.2007.04.017
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发表时间：
2007-08-15
影响因子：
3
通讯作者：
Frouin, Vincent
中科院分区：
文献类型：
--
作者：
Dauguet, Julien;Delzescaux, Thieffy;Frouin, Vincent
尸检数据和活体脑图像之间的相关性对于研究神经退行性疾病具有高度的意义。本文描述了一种协议，匹配一系列的狒狒大脑的染色组织切片与解剖MRI扫描相同的主题，使用中间的3D一致的体积的“blockface”的照片在切片过程中。狒狒大脑的每个染色的组织切片首先使用新的“半刚性”变换与其相应的块面照片相匹配。这种分段刚性2D变换特别适用于包含两个半球的切片的配准。随后，为了校正由组织学制备和固定引起的大脑的全局3D变形，在块面体积和MRI数据之间估计3D弹性变换。然后将该3D弹性变换应用于先前使用半刚性方法对齐的组织学体积，以完成一系列染色的组织学切片与MRI数据的配准。我们通过评估解剖特征的匹配质量以及MRI和组织学图像之间体积测量的差异来评估我们的方法的有效性。两个完整的狒狒大脑（小脑除外）成功地处理使用我们的协议。(C)2007 Elsevier B.V.保留所有权利。
The correlation between post-mortem data and in-vivo brain images is of high interest for studying neurodegenerative diseases. This paper describes a protocol that matches a series of stained histological slices of a baboon brain with an anatomical MRI scan of the same subject using an intermediate 3D-consistent volume of "blockface" photographs taken during the sectioning process. Each stained histological section of the baboon brain was first registered to its corresponding blockface photograph using a novel "hemi-rigid" transformation. This,piecewise rigid 2D transformation was specifically adapted to the registration of slices which contained both hemispheres. Subsenquently, to correct the global 3D deformations of the brain caused by histological preparation and fixation, a 3D elastic transformation was estimated between the blockface volume and the MRI data. This 3D elastic transformation was then applied to the histological volume previously aligned using the hemi-rigid method to complete the registration of the series of stained histological slices with the MRI data. We assessed the efficacy of our method by evaluating the quality of matching of anatomical features as well as the difference of volume measurements between the MRI and the histological images. Two complete baboon brains (with the exception of cerebellum) were successfully processed using our protocol. (C) 2007 Elsevier B.V. All rights reserved.