Molecular components of the signal sequence that function in the initiation of protein export.
Molecular components of the signal sequence that function in the initiation of protein export.
复制标题
在蛋白质输出启动中发挥作用的信号序列的分子成分。
DOI：
10.1083/jcb.95.3.689
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发表时间：
1982-12
影响因子：
7.8
通讯作者：
Silhavy, T J
中科院分区：
文献类型：
--
作者：
Emr, S D;Silhavy, T J
我们正在研究LamB蛋白出口到大肠杆菌外膜的机制。使用基于基因融合的两种选择程序，我们已经确定了一些导致LamB信号序列改变的突变。突变株的特征表明，尽管许多突变使羔羊出口受阻95%以上，但其他突变基本上没有影响。这些结果允许分析信号序列的各种分子成分所执行的功能。我们的研究结果表明，四个氨基酸的关键子集包含在LamB信号序列的中心疏水核心内。如果这个核心可以呈现出α -螺旋构象，这四个氨基酸组成了一个识别位点，与细胞输出机制的一个组成部分相互作用。由于蛋白质定位的机制在进化过程中似乎是保守的，这些结果所建立的原则应该适用于真核细胞的类似研究。
We are studying the mechanism by which the LamB protein is exported to the outer membrane of Escherichia coli. Using two selection procedures based on gene fusions, we have identified a number of mutations that cause alterations in the LamB signal sequence. Characterization of the mutant strains revealed that although many such mutations block LamB export to greater than 95%, others have essentially no effect. These results allow an analysis of the functions performed by the various molecular components of the signal sequence. Our results suggest that a critical subset of four amino acids is contained within the central hydrophobic core of the LamB signal sequence. If this core can assume an alpha-helical conformation, these four amino acids comprise a recognition site that interacts with a component of the cellular export machinery. Since mechanisms of protein localization appear to have been conserved during evolution, the principles established by these results should be applicable to similar studies in eukaryotic cells.