Line narrowing in methyl-TROSY using zero-quantum 1H-13C NMR spectroscopy
Line narrowing in methyl-TROSY using zero-quantum 1H-13C NMR spectroscopy
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DOI：
10.1021/ja039732s
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发表时间：
2004-04-21
影响因子：
15
通讯作者：
Kay, LE
中科院分区：
文献类型：
--
作者：
Tugarinov, V;Sprangers, R;Kay, LE
提出了一种灵敏度提高的零量子关联实验，用于记录高氘代、甲基质子化大分子蛋白质中甲基的H-1-C-13关联。零量子光谱受益于TROSY效应，其中甲基内和甲基间偶极弛豫相互作用最小化。苹果酸合成酶G在5 ℃（81 kDa的单多肽链酶，相关时间为118 ns）和赖氨酸脱羧酶在45 ℃（810 kDa的十聚体酶）的应用表明，相对于相应的HMQC数据集，分辨率有显著提高，灵敏度仅略有下降（接近10%）。
An enhanced sensitivity zero-quantum correlation experiment is proposed for recording H-1-C-13 correlations of methyl groups in highly deuterated, methyl protonated large proteins. The zero-quantum spectra benefit from TROSY-effects in which both intra- and inter-methyl dipolar relaxation interactions are minimized. Applications to malate synthase G at 5degreesC (81 kDa single polypeptide chain enzyme, correlation time of 118 ns) and lysine decarboxylase at 45degreesC (810 kDa decameric enzyme) are presented showing significant improvements in resolution relative to corresponding HMQC data sets, with only slight decreases (similar to10%) in sensitivity.