Expression of peroxisome proliferator-activated receptor γ (PPARγ) in normal human pancreatic islet cells
Expression of peroxisome proliferator-activated receptor γ (PPARγ) in normal human pancreatic islet cells
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DOI：
10.1007/s001250051508
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发表时间：
2000-09-01
期刊：
影响因子：
8.2
通讯作者：
Lefebvre, J
中科院分区：
文献类型：
--
作者：
Dubois, M;Pattou, F;Lefebvre, J
目的/假设。据报道，噻唑烷二酮类可改善啮齿类动物的胰岛形态和β细胞功能，支持噻唑烷二酮类对内分泌胰岛细胞直接作用的假设。在这项研究中，我们研究了表达的过氧化物酶体增殖物激活受体γ，核受体，激活天然存在的脂肪酸和合成噻唑烷二酮，在正常人内分泌胰腺细胞。从10个脑死亡的瘦的非糖尿病成年供体中收获的胰腺分离人胰岛。我们分析了人过氧化物酶体增殖物激活受体γ的基因和蛋白表达，并评估了过氧化物酶体增殖物激活受体γ激动剂对人胰岛制剂中胰岛素分泌的影响。对来自四种不同的人胰岛制剂的总RNA进行的RT-PCR证明了过氧化物酶体增殖物激活受体γ mRNA的存在。Western blot分析显示过氧化物酶体增殖物激活受体γ蛋白的一致表达。过氧化物酶体增殖物激活受体γ被证明是存在于所有三种内分泌细胞类型的研究（α，β和δ细胞）通过免疫组化。我们发现过氧化物酶体增殖物激活受体γ在人胰岛内分泌细胞中在mRNA和蛋白水平上高度表达。这些结果支持过氧化物酶体增殖物激活受体γ激动剂对人胰腺内分泌细胞的直接影响的假设。
Aims/hypothesis. Thiazolidinediones are reported to improve pancreatic islet morphology and beta-cell function in rodents, supporting the hypothesis of a direct action of thiazolidinediones on endocrine islet cells. In this study we examined the expression of the peroxisome proliferator-activated receptor gamma, a nuclear receptor that is activated by naturally occurring fatty acids and synthetic thiazolidinediones, in normal human endocrine pancreatic cells.Methods. Human islets were isolated from pancreata harvested in ten brain-dead lean non-diabetic adult donors. We analysed the gene and protein expression of the human peroxisome proliferator-activated receptor gamma and evaluated the effects of peroxisome proliferator-activated receptor gamma agonist on insulin secretion in human islet preparations,Results. The RT-PCR carried out on total RNA from four distinct human islet preparations demonstrated the presence of peroxisome proliferator-activated receptor gamma mRNA. Western blot analysis showed the consistent expression of peroxisome proliferator-activated receptor gamma protein. Peroxisome proliferator-activated receptor gamma was shown to be present in all three endocrine cell types studied (alpha, beta and delta cells) by immunohistochemistry.Conclusion/interpretation. We found that peroxisome proliferator-activated receptor gamma is highly expressed in human islet endocrine cells, both at the mRNA and protein levels. These results support the hypothesis of a direct influence of peroxisome proliferator-activated receptor gamma agonist on human pancreatic endocrine cells.