Mass Spectrometry for Cellular and Tissue Analyses in a Very Small Region
Mass Spectrometry for Cellular and Tissue Analyses in a Very Small Region
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DOI：
10.2116/analsci.27.163
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发表时间：
2011-02
影响因子：
1.6
通讯作者：
N. Tsuyama;H. Mizuno;T. Masujima
中科院分区：
文献类型：
--
作者：
N. Tsuyama;H. Mizuno;T. Masujima
生物样品的分析现在有望从多细胞分析转向单细胞分析，以精确地了解分子和细胞行为。已经开发了用于澄清细胞或非常小区域中的分子谱的质谱方法。这些技术包括基质辅助激光解吸电离成像质谱（MALDI-IMS）、二次离子质谱（西姆斯）、单细胞MALDI和活单细胞质谱。本文对这些方法进行了综述，并讨论了它们的特点，讨论了这些方法是如何实现我们的目标的。
The analysis of biological specimens is now expected to shift from multi-cell analysis to single cells to know about the molecular and cellular behavior precisely. Mass spectrometric approaches to clarify molecular profiles in a cell or very small region have been developed. Such techniques include matrix-assisted laser desorption ionization imaging mass spectrometry (MALDI-IMS), secondary-ion mass spectrometry (SIMS), single-cell MALDI and live single-cell mass spectrometry. In this paper, these methods are reviewed, and their characteristics are discussed concerning how these methods achieve our aim.