Depth-varying density and organization of chondrocytes in immature and mature bovine articular cartilage assessed by 3D imaging and analysis
Depth-varying density and organization of chondrocytes in immature and mature bovine articular cartilage assessed by 3D imaging and analysis
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DOI：
10.1369/jhc.4a6511.2005
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发表时间：
2005-09-01
影响因子：
3.2
通讯作者：
Sah, RL
中科院分区：
文献类型：
--
作者：
Jadin, KD;Wong, BL;Sah, RL
关节软骨是一种异质性组织，其细胞密度和组织结构随着距离表面的深度而变化。本研究的目的是建立一种方法，用于在软骨的三维（313）图像中定位单个细胞，并量化不同生长阶段细胞结构和细胞组织的深度相关变化。核定位的准确性很高，相对于手动定位的灵敏度为99%。细胞构成（百万细胞/cm（3））分别从胎儿、小牛和成人样本关节面附近的290、310和150减少到1.0 mm深度处的120、110和50。与最近相邻细胞的距离/角度分别为7.9 μ m/31度、7.1 μ m/31度、和9.1 μ m/31度，分别增加/减少到11.6 μ m/31度，12.0 μ m/30度，和19.2 μ m/ 25度，深度为0.7 mm。这里描述的方法可用于分析生长、成熟、老化退化和再生
Articular cartilage is a heterogeneous tissue, with cell density and organization varying with depth from the surface. The objectives of the present study were to establish a method for localizing individual cells in three-dimensional (313) images of cartilage and quantifying depth-associated variation in cellularity and cell organization at different stages of growth. Accuracy of nucleus localization was high, with 99% sensitivity relative to manual localization. Cellularity (million cells per cm(3)) decreased from 290, 310, and 150 near the articular surface in fetal, calf, and adult samples, respectively, to 120, 110, and 50 at a depth of 1.0 mm. The distance/angle to the nearest neighboring cell was 7.9 mu m/31 degrees, 7.1 mu m/31 degrees, and 9.1 mu m/31 degrees for cells at the articular surface of fetal, calf, and adult samples, respectively, and increased/decreased to 11.6 mu m/31 degrees, 12.0 mu m/30 degrees, and 19.2 mu m/ 25 degrees at a depth of 0.7 mm. The methodologies described here may be useful for analyzing the 3D cellular organization of cartilage during growth, maturation, aging, degeneration, and regeneration.