Differential expression of toll-like receptors 2 and 4 in tissues of the human female reproductive tract
Differential expression of toll-like receptors 2 and 4 in tissues of the human female reproductive tract
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DOI：
10.1128/iai.72.10.5799-5806.2004
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发表时间：
2004-10-01
影响因子：
3.1
通讯作者：
Guyre, PM
中科院分区：
文献类型：
--
作者：
Pioli, PA;Amiel, E;Guyre, PM
Toll样受体（TLR）信号转导是机体对病原体攻击的天然免疫应答的重要组成部分。虽然最近的研究已经开始阐明人类女性生殖道组织中获得性免疫的差异，但关于先天防御机制的工作相对较少。我们研究了人类女性生殖道组织中TLR mRNA和蛋白的表达。在输卵管、子宫内膜、宫颈和外宫颈中观察到TLRs 1至6的组成型mRNA表达。此外，在所有测定的组织中也检测到信号衔接子MyD88和辅助分子CD14的转录物。TLR2 mRNA水平的定量分析显示，该分子在输卵管和宫颈组织中的表达最高，其次是子宫内膜和子宫颈外端。与TLR2相反，TLR4的表达沿着尿道逐渐下降，在上部组织（输卵管和子宫内膜）中表达最高，其次是宫颈和外宫颈。除了mRNA，TLR2和TLR4的蛋白质表达也记录在这些组织中。这些数据表明，TLR在女性生殖道的不同隔室中差异表达，并可能提供有关该道内炎症和免疫调节的见解。
Toll-like receptor (TLR) signal transduction is a central component of the innate immune response to pathogenic challenge. Although recent studies have begun to elucidate differences in acquired immunity in tissues of the human female reproductive tract, there is a relative paucity of work regarding innate defense mechanisms. We investigated TLR mRNA and protein expression in tissues of the human female reproductive tract. Constitutive mRNA expression of TLRs 1 to 6 was observed in fallopian tubes, uterine endometrium, cervix, and ectocervix. Furthermore, transcripts of the signaling adapter MyD88 and the accessory molecule CD14 were also detected in all tissues assayed. Quantitative analysis of TLR2 mRNA levels revealed highest expression of this molecule in fallopian tube and cervical tissues, followed by endometrium and ectocervix. In contrast to TLR2, TLR4 expression declined progressively along the tract, with highest expression in the upper tissues (fallopian tubes and endometrium), followed by cervix and ectocervix. In addition to mRNA, protein expression of TLR2 and TLR4 was also documented in these tissues. These data suggest that TLRs are differentially expressed in distinct compartments of the female reproductive tract and may provide insight regarding the regulation of inflammation and immunity within the tract.