Maintaining live cells and tissue slices in the imaging setup.
Maintaining live cells and tissue slices in the imaging setup.
复制标题
在成像设置中维护活细胞和组织切片。
DOI：
10.1101/pdb.top105
复制
发表时间：
2011
影响因子：
--
通讯作者：
Kurpius,Dana
中科院分区：
文献类型：
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作者：
Dailey,MichaelE;Marrs,GlenS;Kurpius,Dana
实时成像为研究动态细胞事件提供了令人兴奋的机会，因为它们发生在真实的时间。然而，这样的实验程序提出了几个挑战。本章讨论了在离体成像过程中维护活体生物样本的一些主要考虑因素，并提出了一些相对简单，低成本的解决方案，以保持样本的健康。
Live imaging provides exciting opportunities to study dynamic cellular events as they occur in real time. However, such experimental procedures present several challenges. This chapter discusses some of the major considerations relating to the maintenance of live biological samples during ex vivo imaging and presents some relatively simple, low-cost solutions to keeping samples healthy.