Gallic acid induces apoptosis in human cervical epithelial cells containing human papillomavirus type 16 episomes
Gallic acid induces apoptosis in human cervical epithelial cells containing human papillomavirus type 16 episomes
复制标题
没食子酸诱导含有人乳头瘤病毒16型附加体的人宫颈上皮细胞凋亡
DOI：
10.1002/jmv.24291
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发表时间：
2016-01-01
影响因子：
12.7
通讯作者：
Chen, Jason J.
中科院分区：
文献类型：
--
作者：
Shi, Lin;Lei, Yanjun;Chen, Jason J.
感染肛门生殖道的高危型人乳头瘤病毒（HPV）与宫颈癌的发生密切相关，宫颈癌是全球女性第二大常见癌症。目前还没有专门针对HPV的治疗药物。多酚类化合物由于其对多种癌症和某些病毒的细胞毒性作用而获得了相当大的关注。在这项研究中，我们研究了几种多酚对人宫颈癌细胞和含有稳定的HPV 16型附加体（HPVep）的人宫颈上皮细胞的细胞增殖和死亡的影响。结果表明，三种多酚类物质对HeLa细胞增殖有抑制作用，且呈剂量依赖性。此外，一个检查的多酚，没食子酸（GA），也抑制HPVep细胞的增殖，并表现出显着的特异性对HPV阳性细胞。GA对HPVep和HeLa细胞的抗增殖作用与细胞凋亡和p53上调有关。这些结果表明，GA可以是一个潜在的候选人的发展，抗HPV药物。J. Med. Virol. 88：127-134，2016. (c)2015 Wiley Periodicals，Inc.
The high-risk human papillomaviruses (HPV) that infect the anogenital tract are strongly associated with the development of cervical carcinoma, which is the second most common cancer in women worldwide. Therapeutic drugs specifically targeting HPV are not available. Polyphenolic compounds have gained considerable attention because of their cytotoxic effects against a variety of cancers and certain viruses. In this study, we examined the effects of several polyphenols on cellular proliferation and death of the human cervical cancer cells and human cervical epithelial cells containing stable HPV type 16 episomes (HPVep). Our results show that three polyphenols inhibited proliferation of HeLa cells dose-dependently. Furthermore, one of the examined polyphenols, gallic acid (GA), also inhibited the proliferation of HPVep cells and exhibited significant specificity towards HPV-positive cells. The anti-proliferative effect of GA on HPVep and HeLa cells was associated with apoptosis and upregulation of p53. These results suggest that GA can be a potential candidate for the development of anti-HPV agents. J. Med. Virol. 88:127-134, 2016. (c) 2015 Wiley Periodicals, Inc.