Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs
Array comparative genomic hybridization with cyanin cis-platinum-labeled DNAs
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DOI：
10.2144/04371dd03
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发表时间：
2004-07-01
期刊：
影响因子：
2.7
通讯作者：
Tanke, HJ
中科院分区：
文献类型：
--
作者：
Raap, AK;van der Burg, MJM;Tanke, HJ
与鸟嘌呤的N7原子反应的荧光顺铂化合物可用于在荧光杂交应用中标记核酸。在这里，我们报告说，花青苷（CyN）的顺铂标记的DNA样品的阵列比较基因组杂交（arrayCGH）可以实现可重复性和可靠性。我们证明，在测试和参考DNA样品中，CyN 3-和CyN 5-顺铂的所有核苷酸的约1%的标记程度产生arrayCGH信号与背景的比率范围从30到40。在小鼠和人肿瘤样品的分析过程中获得的arrayCGH结果与使用酶标记获得的结果相当。因此，我们得出结论，Cy-cis-platinum标记是一种替代酶标记arrayCGH。
Fluorescent cis-platinum compounds that react with the N7 atom of guanine are useful for labeling nucleic acids in fluorescence hybridization applications. Here we report that cyanin (CyN) cis-platinum labeling of DNA samples for array comparative genomic hybridizations (arrayCGH) can be achieved reproducibly and reliably. We demonstrate that degrees of labeling of approximately 1% of all nucleotides in test and reference DNA samples with CyN3- and CyN5-cis-platinum produces arrayCGH signal-to-background ratios ranging from 30 to 40. The arrayCGH results achieved during analyses of mouse and human tumor samples were comparable to those achieved using enzymatic labeling. Thus, we conclude that Cy-cis-platinum labeling is an alternative to enzymatic labeling for arrayCGH.