Fibroblast growth factor levels in the whole embryo and limb bud during chick development.
Fibroblast growth factor levels in the whole embryo and limb bud during chick development.
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DOI：
10.1016/0012-1606(88)90265-5
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发表时间：
1988-07
影响因子：
2.7
通讯作者：
J. Seed;B. Olwin;S. Hauschka
中科院分区：
文献类型：
--
作者：
J. Seed;B. Olwin;S. Hauschka
在未受精卵的蛋黄和蛋清，以及第2.5天（第18期）-13天的鸡胚胎的肢芽和体中，使用两种互补且高度敏感的生物学试验检测到一种与成纤维细胞生长因子（FGF）非常相似的生长因子——125i - A -FGF与3T3细胞的FGF受体结合的竞争，以及刺激MM14细胞（一种依赖于FGF增殖的永久性小鼠骨骼肌细胞系）的DNA合成。进一步证明该生长因子与FGF相似的证据是，当材料与肝素- sepharose柱结合并在2.5MNaCl洗脱后恢复时，生物活性丧失；第12天胚胎的2.5MNaCl部分含有几个表观分子量为12,500-17,500的多肽。鸡胚体中FGF的水平在第2.5天至第6天（18-29期）相当稳定，在1 ~ 2 ng FGF/mg蛋白质之间；但此后水平增加，到第13天，体内含有约15纳克FGF/毫克蛋白质。相反，直到第5天（第27期），肢体芽中的FGF水平高于身体其他部位；然后在第6天和第7天之间经历短暂的下降，之后它会增加，但仍低于身体其他部位观察到的水平。
A growth factor with properties very similar to fibroblast growth factor (FGF) was detected in the yolk and white of unfertilized chick eggs, and in the limb buds and bodies of Day 2.5 (stage 18)-13 chick embryos using two complementary and highly sensitive biological assays—competition of125I-a-FGF binding to the FGF receptors of 3T3 cells and stimulation of DNA synthesis in MM14 cells, a permanent mouse skeletal muscle cell line that is dependent upon FGF for proliferation. Further evidence of the similarity of this growth factor to FGF is provided by the finding that biological activity is lost when the material is bound to a heparin-Sepharose column and restored upon elution with 2.5MNaCl; the 2.5MNaCl fraction from Day 12 embryos contains several polypeptides of apparent molecular weights 12,500–17,500. The level of FGF in the embryonic chick body is fairly constant between Days 2.5 and 6 (stages 18–29), ranging between 1 and 2 ng FGF/mg protein; but thereafter the level increases so that by Day 13 the body contains about 15 ng FGF/mg protein. In contrast, the level of FGF in the limb bud is higher than that in the rest of the body until Day 5 (stage 27); it then undergoes a transient decrease between Days 6 and 7, after which it increases but remains below the level observed in the remainder of the body.