The high integration and differentiation potential of autologous neural stem cell transplantation compared with allogeneic transplantation in adult rat hippocampus
The high integration and differentiation potential of autologous neural stem cell transplantation compared with allogeneic transplantation in adult rat hippocampus
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DOI：
10.1016/j.expneurol.2005.12.004
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发表时间：
2006-06-01
影响因子：
5.3
通讯作者：
Date, I.
中科院分区：
文献类型：
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作者：
Muraoka, K.;Shingo, T.;Date, I.
细胞疗法被认为在旨在恢复受损神经系统功能的恢复性疗法中起着核心作用。本研究的目的是建立一个自体神经干细胞（NSC）移植模型，使用成年大鼠，并比较该系统和异基因NSC移植之间的存活，迁移和分化。此外，我们还比较了自体和异体移植后宿主组织的免疫反应。使用立体定向方法从成年Fischer 344大鼠的室下区取出神经干细胞。将神经干细胞扩增并显微注射到自体脑中的正常海马中。同种异体NSC（来自成年Wistar大鼠）移植使用相同的程序进行，并在移植后3周对海马切片进行免疫组织学分析。自体移植的细胞存活率和迁移率高于异基因移植，自体移植细胞的神经元分化率远高于异基因移植。此外，与同种异体移植相比，自体移植的宿主组织中星形胶质细胞和小胶质细胞的反应性较低。这些发现表明，宿主组织的免疫反应性强烈影响CNS中的细胞移植，因为自体移植不诱导宿主组织免疫反应性;微环境基本上维持在移植细胞的最佳条件下。(c)2005年爱思唯尔公司All rights reserved.
Cell therapy is thought to have a central role in restorative therapy, which aims to restore function to the damaged nervous system. The purpose of this study was to establish an autologous neural stem cell (NSC) transplantation model using adult rats and to compare survival, migration, and differentiation between this system and allogeneic NSC transplantation. Furthermore, we compared the immunologic response of the host tissue between autologous and allogeneic transplantation. NSCs were removed from the subventricular zone of adult Fischer 344 rats using stereotactic methods. NSCs were expanded and microinjected into normal hippocampus in the autologous brain. Allogeneic NSC (derived from adult Wistar rats) transplantation was performed using the same procedure, and hippocampal sections were analyzed immumohistologically 3 weeks post-transplantation. The cell survival and migration rate were higher for autologous transplantation than for allogeneic transplantation, and the neuronal differentiation rate in the autologous transplanted cells far exceeded that of allogeneic transplantation. Furthermore, there was less astrocyte and microglia reactivity in the host tissue of the autologous transplantation compared with allogeneic transplantation. These findings demonstrate that immunoreactivity of the host tissue strongly influences cell transplantation in the CNS as the autologous transplantation did not induce host tissue immunoreactivity; the microenvironment was essentially maintained in an optimal condition for the transplanted cells. (c) 2005 Elsevier Inc. All rights reserved.