ASSOCIATION OF POLIOVIRAL PROTEINS OF THE P2-GENOMIC REGION WITH THE VIRAL REPLICATION COMPLEX AND VIRUS-INDUCED MEMBRANE SYNTHESIS AS VISUALIZED BY ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY AND AUTORADIOGRAPHY
ASSOCIATION OF POLIOVIRAL PROTEINS OF THE P2-GENOMIC REGION WITH THE VIRAL REPLICATION COMPLEX AND VIRUS-INDUCED MEMBRANE SYNTHESIS AS VISUALIZED BY ELECTRON-MICROSCOPIC IMMUNOCYTOCHEMISTRY AND AUTORADIOGRAPHY
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DOI：
10.1016/0042-6822(87)90063-8
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发表时间：
1987-09-01
期刊：
影响因子：
3.7
通讯作者：
PASAMONTES, L
中科院分区：
文献类型：
--
作者：
BIENZ, K;EGGER, D;PASAMONTES, L
使用高分辨率电子显微镜放射自显影和免疫细胞化学与单克隆抗体对脊髓灰质炎病毒蛋白的P2基因组区域，这些蛋白质的位置在病毒诱导的囊泡形成和病毒RNA的合成，随后在病毒复制周期。研究发现，P2蛋白在合成后不久就与rER结合。在蛋白质和rER相互作用的位点，出现电子致密的斑块。同时，膜突起生长并形成囊泡，囊泡最终发芽，在其外表面上携带补丁。如放射自显影所示，这些斑块是病毒RNA复制的位点，因此，它们代表脊髓灰质炎病毒复制复合体。具有复制复合物的囊泡，包括复制和复制的病毒RNA，远离rER，在感染细胞的中心形成连续生长的囊泡区域，在那里发生病毒成熟。2C蛋白的一个可能的功能是将复制复合物或其某些组分附着到囊泡膜上。
Using high resolution electron microscopic autoradiography and immunocytochemistry with monoclonal antibodies against poliovirus proteins of the P2 genomic region, the location of these proteins in respect to the virus-induced vesicle formation and the viral RNA synthesis was followed during the viral replication cycle. It was found that P2 proteins become rER associated soon after their synthesis. At the site of protein and rER interaction, electron-dense patches appear. Simultaneously, membrane protrusions grow and form vesicles which finally budd off, carrying the patches on their outer surface. As shown by autoradiography, these patches are the site of viral RNA replication and, therefore, they represent the poliovirus replication complex. The vesicles with the replication complex, including replicating and replicated viral RNA, move away from the rER to form a continuously growing vesiculated area in the center of the infected cell, where virus maturation takes place. A likely function of the 2C protein is to attach the replication complex, or some of its components, to the vesicular membranes.