Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.
Heteromannan and Heteroxylan Cell Wall Polysaccharides Display Different Dynamics During the Elongation and Secondary Cell Wall Deposition Phases of Cotton Fiber Cell Development.
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DOI：
10.1093/pcp/pcv101
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发表时间：
2015-09
影响因子：
4.9
通讯作者：
Knox JP
中科院分区：
文献类型：
--
作者：
Hernandez-Gomez MC;Runavot JL;Guo X;Bourot S;Benians TA;Willats WG;Meulewaeter F;Knox JP
非纤维素多糖在棉纤维发育中的作用知之甚少。将聚糖微阵列和原位分析与单克隆抗体、多糖连接分析和转录谱分析相结合，研究了发育和成熟棉花（Gossypium spp.）纤维已经确定。对棉花花后不同时期纤维的比较分析表明，异甘露聚糖和异木聚糖在纤维发育过程中具有不同的时空调控规律。LM 21杂甘露聚糖表位在纤维伸长阶段更加丰富，主要定位在初生细胞壁中。相比之下，AX1杂木聚糖表位发生在过渡阶段和次生细胞壁沉积期间，并定位在棉纤维的初生和次生细胞壁中。这些发育动力学的生物合成基因的转录谱的支持。而我们的数据表明，在纤维伸长的作用，heteroxylan是可能参与调节次生细胞壁的纤维素沉积。此外，在纤维发育过程中，这些表位的相对丰度在不同的棉花品系之间变化，这些品系具有来自四个物种（G。陆地棉G. n. barbadense，海岛棉G. arboreum和G. herbaceum），表明这些非纤维素多糖可能参与决定最终纤维质量和工业加工的适用性。
The roles of non-cellulosic polysaccharides in cotton fiber development are poorly understood. Combining glycan microarrays and in situ analyses with monoclonal antibodies, polysaccharide linkage analyses and transcript profiling, the occurrence of heteromannan and heteroxylan polysaccharides and related genes in developing and mature cotton (Gossypium spp.) fibers has been determined. Comparative analyses on cotton fibers at selected days post-anthesis indicate different temporal and spatial regulation of heteromannan and heteroxylan during fiber development. The LM21 heteromannan epitope was more abundant during the fiber elongation phase and localized mainly in the primary cell wall. In contrast, the AX1 heteroxylan epitope occurred at the transition phase and during secondary cell wall deposition, and localized in both the primary and the secondary cell walls of the cotton fiber. These developmental dynamics were supported by transcript profiling of biosynthetic genes. Whereas our data suggest a role for heteromannan in fiber elongation, heteroxylan is likely to be involved in the regulation of cellulose deposition of secondary cell walls. In addition, the relative abundance of these epitopes during fiber development varied between cotton lines with contrasting fiber characteristics from four species (G. hirsutum, G. barbadense, G. arboreum and G. herbaceum), suggesting that these non-cellulosic polysaccharides may be involved in determining final fiber quality and suitability for industrial processing.