Conditional depletion of nuclear proteins by the Anchor Away system.
Conditional depletion of nuclear proteins by the Anchor Away system.
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DOI：
10.1002/0471142727.mb1310bs93
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发表时间：
2011-01-01
影响因子：
--
通讯作者：
Jin, Yi
中科院分区：
文献类型：
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作者：
Fan, Xiaochun;Geisberg, Joseph V;Jin, Yi
核蛋白在许多重要的细胞过程的调节中起着关键作用。在酿酒酵母中，许多编码核蛋白的基因是必需的。该单元描述了一种称为锚离的方法，该方法可用于有条件地快速消耗感兴趣的核蛋白。它涉及感兴趣的蛋白质有条件地输出到细胞核外，并随后在细胞质中隔离。该方法可用于同时从细胞核中去除多种蛋白质。
Nuclear proteins play key roles in the regulation of many important cellular processes. In Saccharomyces cerevisiae, many genes encoding nuclear proteins are essential. This unit describes a method termed Anchor Away that can be used to conditionally and rapidly deplete nuclear proteins of interest. It involves conditional export of the protein of interest out of the nucleus and its subsequent sequestration in the cytoplasm. This method can be used to simultaneously deplete multiple proteins from the nucleus.