Silk Gland Fibroin from Indian Muga Silkworm Antheraea assama as Potential Biomaterial
Silk Gland Fibroin from Indian Muga Silkworm Antheraea assama as Potential Biomaterial
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DOI：
10.1007/s13770-012-0008-6
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发表时间：
2013-08-01
影响因子：
3.6
通讯作者：
Kundu, S. C.
中科院分区：
文献类型：
--
作者：
Kar, Subrata;Talukdar, Sarmistha;Kundu, S. C.
对新型多功能生物材料的需求日益增加。许多研究人员报道，丝绸是一种理想的生物材料，可用于不同的生物医学应用。大多数研究都是对桑蚕蚕丝进行的，而对印度非桑蚕蚕丝的研究相对较少。本文以非桑蚕Antheraea assama （assamensis）腺的再生水丝素蛋白制备二维基质。评价了其生物化学、生物物理特性及其生物医学用途的细胞相容性。将该蚕腺丝蛋白与非桑蚕印度热带塔沙蚕蚕丝腺丝蛋白及桑蚕蚕茧丝蛋白进行了性能比较。柞蚕的腺丝蛋白由两个约250 kDa的多肽组成，每个多肽由二硫键连接。傅里叶变换红外光谱和x射线衍射研究表明溶解的丝素蛋白溶液具有随机线圈结构。经乙醇处理后，二维薄膜中的α -螺旋结构变为β -薄片，具有结晶性和不溶性。丝素蛋白膜的亲水性最差，其次是家蚕丝和蚕丝。通过MG-63人成骨样细胞的细胞附着和扩散研究，研究了这三种生物膜的生物相容性。非桑纤维蛋白基质的细胞相容性与标准组织培养板相当。结果表明，非桑树印度蚕丝腺丝素也是一种适合用于组织工程的天然生物材料基质。
There is an increasing demand for new versatile biomaterials. Silk is reported by many researchers as an ideal biomaterial for different biomedical applications. Most of the studies are carried out on mulberry silk Bombyx mori, however silk from Indian non-mulberry silkworms are relatively unexplored. In this report we fabricate 2D matrices from the regenerated aqueous silk fibroin protein of the glands of non-mulberry Indian muga silkworm Antheraea assama (assamensis). Its biochemical, biophysical characteristics and its cytocompatibility for biomedical uses are evaluated. The properties of this muga gland fibroin are compared with silk gland fibroin from non-mulberry Indian tropical tasar silkworm Antheraea mylitta and with the fibroin from the cocoon of mulberry silkworm Bombyx mori. The gland fibroin of Antheraea assama is observed to consist of two polypeptides of approximately 250 kDa each linked by disulfide bond. Fourier transformed infrared spectroscopy and X-ray diffraction studies indicate random coil structure of dissolved fibroin solution. The alpha-helical structure in 2D films changed to beta-sheets upon ethanol treatment, imparting crystallinity and insolubility. The fibroin film is found to be the least hydrophilic, followed by B. mori and A. mylitta silk. Biocompatibility of the films from all three species is investigated through the cell attachment and spreading study of MG-63 human osteoblast-like cells. The cytocompatibility of non-mulberry fibroin matrices are comparable with that of standard tissue culture plates. The results indicate that the non-mulberry Indian muga silk gland fibroin is also suitable matrix as a natural biomaterial for tissue engineering applications.