Increased expression of human type IIa secretory phospholipase A2 antigen in arthritic synovium
Increased expression of human type IIa secretory phospholipase A2 antigen in arthritic synovium
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DOI：
10.1136/ard.57.9.550
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发表时间：
1998-09-01
影响因子：
27.4
通讯作者：
Scott, KF
中科院分区：
文献类型：
--
作者：
Jamal, OS;Conaghan, PG;Scott, KF
目的：检测人类IIa分泌型磷脂酶A(2)在类风湿关节炎(RA)、骨关节炎(OA)和非关节炎(NA)患者滑膜中的定位和表达水平，并探讨其与炎症组织学特征的关系。方法采用免疫组织化学方法，对10例RA、10例OA和10例NA患者膝关节滑膜冰冻切片进行免疫组织化学染色。在每例有代表性的组织切片的10个视野中，按0~3分对Spla(2)阳性细胞进行评分。用CD14、CD45和9c1双标记免疫荧光共聚焦显微镜检测细胞类型特异性。通过对衬里厚度和单个核细胞浸润物(MC)的半定量评分以及将这两个参数相加得出的累计炎症评分来评估炎症。采用非参数统计分析比较两组间的得分。结果：Spla(2)在所有组织切片中均定位于内皮细胞(EC)、血管平滑肌(VSM)和肥大细胞(M)。在RA和OA切片中，在滑膜衬里(LL)和滑膜下衬里(SLL)均可见巨噬细胞样细胞和成纤维细胞样细胞染色。神经周细胞染色阳性。内膜下淋巴集合体(LA)在所有切片均为阴性。RA组血管外滑膜组织(中位数3.6，范围1.5~6.0)的染色强度明显高于OA(中位数1.95，范围0~5.3)或NA(中位数0，0~5.9)组(P<0.05)。
Objective-To determine the localisation and level of expression of human type IIa secretory phospholipase A(2) (sPLA(2)) in the synovium of rheumatoid arthritis (RA), osteoarthritis (OA), and non-arthritic (NA) patients and to examine the relation between sPLA(2) and histological features of inflammation.Methods-Immunoperoxidase staining using the anti-sPLA(2) monoclonal antibody 9C1 was performed on frozen sections of knee synovium of 10 RA, 10 OA, and 10 NA patients. sPLA(2) positive cells were scored on a scale of 0-3 in 10 fields of a representative tissue section from each case. Double labelling imunofluorescence confocal microscopy with antibodies to CD14 or CD45 and 9C1 was used to determine cell type specificity. Inflammation was assessed by semiquantitative scoring of lining layer thickness and mononuclear cell infiltrates (MC) and a cumulative inflammation score, generated by summing the two parameters. Scores in each group were compared using nonparametric statistical analysis. Results-sPLA(2) was localised to endothelium (EC), vascular smooth muscle (VSM), and mast cells (M) in all tissue sections. In RA and OA sections, staining was seen in both macrophage-like and fibroblast-like cells in the synovial lining layer (LL) and subsynovial lining layer (SLL). Perineural cells stained positively. Subintimal lymphoid aggregates (LA) were negative in all sections. The RA group showed significantly greater staining in extravascular synovial tissue (median 3.6, range 1.5-6.0) than the OA (median 1.95, range 0-5.3) or NA (median 0, range 0-5.9) groups (p