An Efficient Antioxidant System in a Long-Lived Termite Queen.
An Efficient Antioxidant System in a Long-Lived Termite Queen.
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DOI：
10.1371/journal.pone.0167412
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Iuchi Y
中科院分区：
文献类型：
--
作者：
Tasaki E;Kobayashi K;Matsuura K;Iuchi Y
繁殖和寿命之间的权衡在各种动物中是众所周知的。群居昆虫女王是一种罕见的有机体，可以在不牺牲繁殖力的情况下获得很长的寿命。社会性昆虫蜂王的长寿与这种权衡相矛盾，它引起了人们的广泛关注，因为它暗示着一种非凡的抗衰老机制的存在。在这里，我们表明，白蚁Reticulitermes speratus的女王招致显着降低氧化损伤的DNA，蛋白质和脂质，并具有较高的活性的抗氧化酶比非生殖个体（工人和士兵）。紫外线照射后，蜂王体内的8-羟基-2 '-脱氧鸟苷（DNA氧化损伤标志物）水平低于工蜂。皇后区还显示蛋白质羰基和丙二醛（分别为蛋白质和脂质的氧化损伤标志物）水平较低。昆虫体内的抗氧化酶一般由过氧化氢酶（CAT）和过氧化物氧还蛋白（Prx）组成。皇后的CAT活性是工人的两倍多，CAT基因RsCAT 1的表达水平是工人的七倍多。白蚁蚁后的CAT活性也明显高于其他独居昆虫和真社会性Hyattera蚁后。此外，皇后表现出较高的表达水平的Prx基因RsPRX 6。这些结果表明，这种有效的抗氧化系统可以部分解释为什么白蚁蚁后实现长寿。本研究为了解群居昆虫生殖分工与蚁后抗氧化应激能力的进化联系提供了重要的线索。
The trade-off between reproduction and longevity is known in wide variety of animals. Social insect queens are rare organisms that can achieve a long lifespan without sacrificing fecundity. The extended longevity of social insect queens, which contradicts the trade-off, has attracted much attention because it implies the existence of an extraordinary anti-aging mechanism. Here, we show that queens of the termite Reticulitermes speratus incur significantly lower oxidative damage to DNA, protein and lipid and have higher activity of antioxidant enzymes than non-reproductive individuals (workers and soldiers). The levels of 8-hydroxy-2’-deoxyguanosine (oxidative damage marker of DNA) were lower in queens than in workers after UV irradiation. Queens also showed lower levels of protein carbonyls and malondialdehyde (oxidative damage markers of protein and lipid, respectively). The antioxidant enzymes of insects are generally composed of catalase (CAT) and peroxiredoxin (Prx). Queens showed more than two times higher CAT activity and more than seven times higher expression levels of the CAT gene RsCAT1 than workers. The CAT activity of termite queens was also markedly higher in comparison with other solitary insects and the queens of eusocial Hymenoptera. In addition, queens showed higher expression levels of the Prx gene RsPRX6. These results suggested that this efficient antioxidant system can partly explain why termite queens achieve long life. This study provides important insights into the evolutionary linkage of reproductive division of labor and the development of queens’ oxidative stress resistance in social insects.