The piRNA pathway is essential for generating functional oocytes in golden hamsters
The piRNA pathway is essential for generating functional oocytes in golden hamsters
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DOI：
10.1038/s41556-021-00750-6
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发表时间：
2021-09
影响因子：
21.3
通讯作者：
Hongdao Zhang;Fengjuan Zhang;Qinghua Chen;Mingzhe Li;Xiaolong Lv;Yali Xiao;Zhaozhen Zhang;
中科院分区：
文献类型：
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作者：
Hongdao Zhang;Fengjuan Zhang;Qinghua Chen;Mingzhe Li;Xiaolong Lv;Yali Xiao;Zhaozhen Zhang;
Piwi相互作用RNA（piRNA）主要在生殖细胞中表达，并在各种物种的配子发生中起作用。然而，Piwi缺陷的雌性小鼠是可生育的，并且小鼠卵母细胞表达一组似乎不广泛代表哺乳动物的小RNA。因此，piRNA在哺乳动物卵子发生中的功能在很大程度上仍然不清楚。在这里，我们产生了Piwil 1和Mov 10 l1缺陷型金黄地鼠，发现所有雌性和雄性突变体都是不育的，分别在胚胎发生和精子发生方面存在严重缺陷。在Piwil 1缺陷雌性仓鼠中，卵母细胞和胚胎表现出异常转座子积累和广泛的转录组失调，胚胎停滞在两细胞阶段，合子基因组激活受损。此外，PIWIL 1-piRNA在沉默卵母细胞和胚胎中的内源性逆转录病毒方面发挥非冗余功能。总之，我们的研究结果表明，piRNA对于在金黄仓鼠中产生功能性生殖细胞是不可或缺的，并显示了这种模式物种对于配子发生中的皮尔纳研究的价值，特别是与雌性不育相关的研究。
Piwi-interacting RNAs (piRNAs) are predominantly expressed in germ cells and function in gametogenesis in various species. However,Piwi-deficient female mice are fertile and mouse oocytes express a panel of small RNAs that do not appear to be widely representative of mammals. Thus, the function of piRNAs in mammalian oogenesis remains largely unclear. Here, we generatedPiwil1- andMov10l1-deficient golden hamsters and found that all female and male mutants were sterile, with severe defects in embryogenesis and spermatogenesis, respectively. InPiwil1-deficient female hamsters, the oocytes and embryos displayed aberrant transposon accumulation and extensive transcriptomic dysregulation, and the embryos were arrested at the two-cell stage with impaired zygotic genome activation. Moreover, PIWIL1-piRNAs exert a non-redundant function in silencing endogenous retroviruses in the oocytes and embryos. Together, our findings demonstrate that piRNAs are indispensable for generating functional germ cells in golden hamsters and show the value of this model species for piRNA studies in gametogenesis, especially those related to female infertility.