Slowing DNA translocation in a solid-state nanopore
Slowing DNA translocation in a solid-state nanopore
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DOI：
10.1021/nl051063o
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发表时间：
2005-09-01
期刊：
影响因子：
10.8
通讯作者：
Li, JL
中科院分区：
文献类型：
--
作者：
Fologea, D;Uplinger, J;Li, JL
降低DNA分子在固态纳米孔中的 translocation（易位，此处可能指通过纳米孔的移动过程）速度是实现快速单分子识别的关键一步。在此我们证明，通过控制电解质温度、盐浓度、黏度以及纳米孔两端的偏置电压，DNA的易位速度能够比之前的结果降低一个数量级。我们在直径为4 - 8纳米的氮化硅孔中，获得了3千碱基对双链DNA每秒3个碱基的易位速度。我们的结果还表明，这种纳米孔内部的离子电导率比本体（此处可能指溶液整体）中的要小。
Reducing a DNA molecule's translocation speed in a solid-state nanopore is a key step toward rapid single molecule identification. Here we demonstrate that DNA translocation speeds can be reduced by an order of magnitude over previous results, By controlling the electrolyte temperature, salt concentration, viscosity, and the electrical bias voltage across the nanopore, we obtain a 3 base/mu s translocation speed for 3 kbp double-stranded DNA in a 4-8 nm diameter silicon nitride pore. Our results also indicate that the ionic conductivity inside such a nanopore is smaller than it is in bulk.