Nicotinic acetylcholine receptors in a songbird brain
Nicotinic acetylcholine receptors in a songbird brain
复制标题
DOI：
10.1002/cne.25314
复制
发表时间：
2022-03-28
影响因子：
2.5
通讯作者：
Wada, Kazuhiro
中科院分区：
文献类型：
--
作者：
Asogwa, Norman Chinweike;Toji, Noriyuki;Wada, Kazuhiro
烟碱乙酰胆碱受体（nachr）是一种配体门控离子通道，介导快速突触传递和细胞信号传导，对学习、记忆和运动技能的执行有贡献。鸣禽是一种复杂的习得的运动技能。虽然已经预测鸟类基因组中存在15个nAChR亚基，但它们的表达模式及其对鸣叫学习和产生的潜在贡献尚未得到全面研究。本文克隆了斑胸草雀大脑中所有15个nAChR亚基（ChrnA1-10、B2-4、D和G），并研究了在鸣叫学习的关键时期，负责鸣叫学习和产生的神经通路的mRNA表达模式。虽然没有检测到ChrnA1， A6， A9和A10的杂交信号，但其他11个nAChR亚基在鸣禽脑鸣叫核的一个或多个主要亚基中唯一表达。在这11个亚基中，ChrnA3-5、A7和B2与周围解剖相关区域相比，在歌核中受到差异调节。在歌唱学习的关键时期，前毛外侧大细胞核中ChrnA5表达上调。此外，单细胞RNA测序结果显示，ChrnA7和B2是声带运动核HVC神经元和栉状核强健核中表达的主要亚基，这表明在有限的细胞群体中可能存在ChrnA7-同质和chrnb2 -异质nachr。这些结果表明，相对有限类型的nAChR亚基对鸣禽的鸣叫学习和鸣叫产生有功能贡献。
Nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that mediate fast synaptic transmission and cell signaling, which contribute to learning, memory, and the execution of motor skills. Birdsong is a complex learned motor skill in songbirds. Although the existence of 15 nAChR subunits has been predicted in the avian genome, their expression patterns and potential contributions to song learning and production have not been comprehensively investigated. Here, we cloned all the 15 nAChR subunits (ChrnA1-10, B2-4, D, and G) from the zebra finch brain and investigated the mRNA expression patterns in the neural pathways responsible for the learning and production of birdsong during a critical period of song learning. Although there were no detectable hybridization signals for ChrnA1, A6, A9, and A10, the other 11 nAChR subunits were uniquely expressed in one or more major subdivisions in the song nuclei of the songbird brain. Of these 11 subunits, ChrnA3-5, A7, and B2 were differentially regulated in the song nuclei compared with the surrounding anatomically related regions. ChrnA5 was upregulated during the critical period of song learning in the lateral magnocellular nucleus of the anterior nidopallium. Furthermore, single-cell RNA sequencing revealed ChrnA7 and B2 to be the major subunits expressed in neurons of the vocal motor nuclei HVC and robust nucleus of the arcopallium, indicating the potential existence of ChrnA7-homomeric and ChrnB2-heteromeric nAChRs in limited cell populations. These results suggest that relatively limited types of nAChR subunits provide functional contributions to song learning and production in songbirds.