Weak microwave can alleviate water deficit induced by osmotic stress in wheat seedlings
Weak microwave can alleviate water deficit induced by osmotic stress in wheat seedlings
复制标题
弱微波可缓解渗透胁迫引起的小麦幼苗水分亏缺
DOI：
10.1007/s00425-008-0828-8
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发表时间：
2009-01-01
期刊：
影响因子：
4.3
通讯作者：
Han, Xiao-Ling
中科院分区：
文献类型：
--
作者：
Chen, Yi-Ping;Jia, Jing-Fen;Han, Xiao-Ling
研究了微波预处理小麦种子对小麦幼苗抗渗透胁迫能力的影响。测定了生物物理、生理生化特性的变化。结果显示：（1）微波预处理5、10、15、20、25 s的磁场强度和种子温度均高于对照。虽然每种微波预处理都导致α-淀粉酶活性和光子发射强度的增加，但α-淀粉酶活性和光子发射强度的增加在微波预处理10 s时最大。(2)与对照相比，PEG处理诱导的渗透胁迫使幼苗丙二醛含量增加，一氧化氮合酶、过氧化氢酶、过氧化物酶、超氧化物歧化酶活性以及一氧化氮、抗坏血酸、谷胱甘肽含量降低。但与单独渗透胁迫相比，微波辐射+渗透胁迫处理的幼苗丙二醛含量降低，一氧化氮合酶、过氧化氢酶、过氧化物酶、超氧化物歧化酶活性以及一氧化氮、抗坏血酸和谷胱甘肽含量升高。这些结果表明，适宜剂量的微波辐射能增强小麦幼苗清除渗透胁迫诱导的自由基的能力，从而提高小麦幼苗对渗透胁迫的抗性。
The aim of the investigation is to determine the effect of microwave pretreatment of wheat seeds on the resistance of seedlings to osmotic stress. Changes in biophysical, physiological and biochemical characters were measured. The results showed: (1) The magnetic field intensity and seeds temperature increased progressively with microwave pretreatments of 5, 10, 15, 20 s and 25 s compared with controls. Although each microwave pretreatment resulted in an increase in alpha-amylase activity and photon emission intensity, the increase of alpha-amylase activity and photon emission intensity was maximal at a microwave pretreatment of 10 s. (2) Osmotic stress induced by PEG treatment enhanced the concentration of malondialdehyde, while decreasing the activities of nitricoxide synthase, catalase, peroxidase, superoxide dismutase and the concentration of nitric oxide, ascorbic acid, glutathione in the seedlings compared with controls. However, compared to osmotic stress alone, in the seedlings treated with microwave irradiation plus osmotic stress the concentration of malondialdehyde decreased, while the activities of nitricoxide synthase, catalase, peroxidase, superoxide dismutase and the concentration of nitric oxide, ascorbic acid and glutathione increased. These results suggest that a suitable dose of microwave radiation can enhance the capability to eliminate free radicals induced by osmotic stress in wheat seedlings resulting in an increase in resistance to osmotic stress.