Effect of ABA upon anthocyanin synthesis in regenerated torenia shoots
Effect of ABA upon anthocyanin synthesis in regenerated torenia shoots
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DOI：
10.1007/s10265-005-0256-9
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发表时间：
2006-03-01
影响因子：
2.8
通讯作者：
Ozeki, Y
中科院分区：
文献类型：
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作者：
Nagira, Y;Ikegami, K;Ozeki, Y
为阐明蓝猪耳（Torenia fournieri）花青苷合成诱导与叶绿素降解的机理，建立了蓝猪耳（Torenia fournieri）再生苗花青苷合成与叶绿素降解诱导体系。本文研究了几种植物生长调节剂对蓝猪耳不定芽再生系统中花色素苷合成和叶绿素降解的影响。在低浓度蔗糖（1.5%）的培养基上，外源脱落酸（阿坝）能诱导蓝猪耳再生苗花青苷合成和叶绿素衰老。在含7%蔗糖的培养基上，测定了再生苗花青苷合成过程中内源阿坝含量的变化。结果表明，7%蔗糖处理在诱导花青苷合成和叶绿素降解之前提高了内源阿坝水平。然而，当再转移到1.5%蔗糖培养基中时，阿坝水平逐渐下降，花青素合成诱导失败，正常的芽再生。这些结果表明，内源阿坝含量的变化可能在诱导蓝猪耳再生芽花青苷合成和叶绿素降解中起重要作用。
To elucidate the mechanism of anthocyanin synthesis induction concomitant with chlorophyll degradation, we established a system in which anthocyanin synthesis and degradation of chlorophyll in regenerated torenia (Torenia fournieri) shoots was induced on medium containing 7% sucrose. Here, we studied the effect of several plant-growth regulators on anthocyanin synthesis and the degradation of chlorophyll in the torenia shoot regenerating system. Exogenous abscisic acid (ABA) could induce anthocyanin synthesis and chlorophyll senescence in regenerating torenia shoots on the medium containing a low concentration of sucrose (1.5%). We determined the changes in the amount of endogenous ABA in the regenerated shoots during the process of anthocyanin synthesis on the medium containing 7% sucrose. It was revealed that the 7% sucrose treatment elevated endogenous ABA levels before the induction of anthocyanin synthesis and chlorophyll degradation. However, while retransfer to the 1.5% sucrose medium resulted in a gradual decrease in the ABA level and a failure of induction of anthocyanin synthesis, normal shoot regeneration. These results suggest that changes in the amount of endogenous ABA may play an important role in the induction of anthocyanin synthesis and chlorophyll degradation in regenerated torenia shoots.